177,004 results
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Plasmid#211512PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteriaDepositorInsertSnxA-2xPX
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304_CMV::mCherry-LgBiT-FLAG-NES
Plasmid#199715PurposeExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-LgBiT-FLAG-NES
UseLentiviral and LuciferaseExpressionMammalianPromoterCMVAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cre
Plasmid#198274PurposeFor loading Cre into PVCs; also contains PVC regulatory genesDepositorInsertPdp1_NTD-Cre
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-Giantin_C1
Plasmid#189773PurposeConstruct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusionDepositorInsertmScarlet3-Giantin
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gi2-CASE
Plasmid#168121PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at C112/E115 within GNAI2Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
G13-CASE
Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Soma-jGCaMP8m
Plasmid#169257PurposeAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.DepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertSoma-jGCaMP8m
UseAAVTags6xHis-tagExpressionMammalianAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_eCB2.0
Plasmid#164604PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in neuronsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVMutationS383TPromoterhSynAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK265 (RAD21-mAC Hygro)
Plasmid#140539PurposeRAD21 tagging with mAID-CloverDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dPguCas13b-3xmRuby3-NLS
Plasmid#132406Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralExpressionMammalianMutationH151A; H1121AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-Citron1
Plasmid#134305Purposemitochondria-targeted direct-response citrate sensorDepositorInsertCitron1
ExpressionMammalianPromoterCMVAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2-HuC(elavl3)-CaMPARI2
Plasmid#137185Purposepan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)DepositorInsertHuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
UseTol2 plasmid for zebrafishTagsFLAG, HA, mycPromoterHuC(elavl3)Available SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag
Plasmid#132397Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1058AAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbAW4k-loxP-TT-loxP-mRFP1
Plasmid#134405PurposeFluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.DepositorInsertW4-loxP-TT-loxP-mRFP1
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterW4 (modified T7A1)Available SinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CyanoGate Kit
Plasmid Kit#1000000146PurposeVectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial ExpressionCloning TypeGolden Gate (MoClo)Available SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-beta-HA-BRCA2
Plasmid#133553PurposeMammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORFDepositorInsertBRCA2 (BRCA2 Human)
ExpressionMammalianAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-h-cGAS
Plasmid#127161Purposeexpression of human cGAS proteinDepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon TVA-mCherry
Plasmid#131779PurposeINTRSECT 2.0DepositorInsertTVA-mCherry
UseAAV; IntrsectTagsmCherryExpressionMammalianPromoternEFAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) (AAV2)
Viral Prep#121538-AAV2PurposeReady-to-use AAV2 particles produced from pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) (#121538). In addition to the viral particles, you will also receive purified pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) plasmid DNA. Synapsin-driven, HA-tagged hM4D(Gi) for neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsHAAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only