164,868 results

1. pTara:500

   Plasmid

   #60717

   Purpose

   Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF6-CSPG4-myc-his

   Plasmid

   #69037

   Purpose

   Expression of CSPG4 in mammalian cells

   Depositor

   Wensheng Wei

   Article

   Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

   Insert

   CSPG4 (CSPG4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1 alpha

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Beta-arrestin2 GFP WT

   Plasmid

   #35411

   Depositor

   Robert Lefkowitz

   Article

   Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

   Insert

   βarrestin2 (Arrb2 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-RPS6 (pDD1902)

   Plasmid

   #52913

   Purpose

   contains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.

   Depositor

   Dirk Dittmer

   Article

   Chen et al J Virol. 2011 Sep;85(18):9495-505. doi:

   Insert

   HAS6 (RPS6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCW-HA-hRb-delta-CDK-puro

   Plasmid

   #212673

   Purpose

   Express tagged hRb delta-CDK phosphosite mutant

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   All 15 CDK phospho-sites are mutated to alanines

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK-2-Smad2-3'UTR

   Plasmid

   #61775

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Smad2

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Smad2 3'UTR
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

7. mTagGFP2-Clathrin-15

   Plasmid

   #56413

   Purpose

   Localization: Clathrin Vesicles, Excitation: 483, Emission: 506

   Depositor

   Michael Davidson

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   mTagGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

   Plasmid

   #67980

   Purpose

   Cas9 activity reporter with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQC membrane TdTomato IX

   Plasmid

   #37351

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   membrane TdTomato
   

   Use

   Tags

   Palmitoylation signal*

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mEGFP-H4-23

    Plasmid

    #56463

    Purpose

    Localization: Nucleus/Histones, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    H4 (HIST1H4J Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pLX303

    Plasmid

    #25897

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. aP2-luciferase

    Plasmid

    #8858

    Depositor

    Bruce Spiegelman

    Article

    Graves et al Mol Cell Biol. 1992 Mar . 12(3):1202-8.

    Insert

    aP2 enhancer / promoter (Fabp4 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase reporter

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mRuby2-pBAD

    Plasmid

    #54771

    Purpose

    Localization: Protein Expression Vector , Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    6xHis and mRuby2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA3 p53 S15A

    Plasmid

    #69004

    Purpose

    mammalian expression of human p53 S15A mutant.

    Depositor

    David Meek

    Article

    Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S15A

    Promoter

    CMV

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IDG_TMEM63B_OE_1

    Plasmid

    #161682

    Purpose

    Overexpresses TMEM63B

    Depositor

    Mike McManus

    Insert

    TMEM63B (TMEM63B Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMevT

    Plasmid

    #17815

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPD1101 Integration Test Circuit Vector

    Plasmid

    #139251

    Purpose

    Integration vector with TUs incorporated. Cells will be red and, if treated with doxycycline, blue.

    Depositor

    Joshua Leonard

    Insert

    Tus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-CamKIIa-jGCaMP8m-WPRE

    Plasmid

    #176751

    Purpose

    AAV-mediated expression of GCaMP8m under the CamKIIa promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIalpha

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcGFP1-N1-SialT

    Plasmid

    #87324

    Purpose

    To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferase

    Depositor

    Lei Lu

    Article

    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

    Insert

    ST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCE-K2N

    Plasmid

    #154879

    Purpose

    Episomally expresses KLF2 and NANOG in mammalian cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

    Insert

    KLF2-2A-NANOG (KLF2, NANOG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge