164,868 results

1. pATetO 6XHis T4 DNA Ligase

   Plasmid

   #206207

   Purpose

   Inducible expression of T4 DNA ligase

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   T4 DNA ligase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-MXT GIRK2

   Plasmid

   #20675

   Depositor

   Henry Lester

   Article

   Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

   Insert

   Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
   

   Use

   Xenopus expression

   Tags

   3' UTR Xenopus B- globin and 5' UTR Xen…

   Expression

   Mutation

   A369V

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. plenti-UbcP-FLAG-CRBN-pGK-HYG

   Plasmid

   #107374

   Purpose

   Lentiviral expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   UbcP

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLeAPS-GFP-blast

   Plasmid

   #182230

   Purpose

   Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

   Depositor

   Michael Birnbaum

   Article

   Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

   Insert

   GFP-P2A-Blasticidin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMU-NUCr

   Plasmid

   #61168

   Purpose

   Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   NLS-mCherry-GUS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hsyn-GRAB_Ado1.0

   Plasmid

   #153285

   Purpose

   Express the adenosine sensor GRAB_Ado1.0 in neurons

   Depositor

   Yulong Li

   Article

   Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

   Insert

   Adenosine sensor GRAB_Ado1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

   Plasmid

   #177919

   Purpose

   Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCX-OKS-2A

   Plasmid

   #19771

   Purpose

   Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Science. 2008 Nov 7. 322(5903):949-53.

   Insert

   Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Three genes are connected with the foot-and-mouth…

   Promoter

   Available since

   Jan. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMV306hsp

    Plasmid

    #26155

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    hsp60 promoter
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDonor14-N7HELIX-KanR

    Plasmid

    #190663

    Purpose

    Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTS-mCherry-GFP1-10-Hyg-N1

    Plasmid

    #91957

    Purpose

    Expresses mCherry-GFP1-10 in mammalian cell mitochondria

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    MTS-mCherry-GFP1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEvol-pAzFRS.1.t1

    Plasmid

    #73547

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.1.t1
    pAzFRS.1.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1-Tspan4

    Plasmid

    #210323

    Purpose

    Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

    Depositor

    Li Yu

    Article

    Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

    Insert

    Tetraspanin 4 (Tspan4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PCBP1_Full-length_pGEX

    Plasmid

    #135108

    Purpose

    Recombinant expression of PCBP1 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    PCBP1 (PCBP1 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRIM3-rfp

    Plasmid

    #74073

    Purpose

    GentR version of pRIM2 carrying rfp

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    rfp
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dyn2-pmCherryN1

    Plasmid

    #27689

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Dynamin 2 (DNM2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRUPATH Triple GaoB

    Plasmid

    #196052

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUGW mCherry-KASH

    Plasmid

    #131505

    Purpose

    mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    mCherry_KASH
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166014

    Purpose

    mammalian expression of HCoV-HKU1 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-HKU1 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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