167,910 results

1. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

   Plasmid

   #118593

   Purpose

   Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha/TRE3G

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1a-eFFly-eGFP

   Plasmid

   #104834

   Purpose

   eFFly luciferase for invivo Imaging, eGFP for flourescent detection

   Depositor

   Irmela Jeremias

   Article

   Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

   Inserts

   eFFly
   eGFP
   

   Use

   Lentiviral and Luciferase; Egfp

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/Puro-CAG-ASAP2s

   Plasmid

   #101274

   Purpose

   Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBMN mEGFP-OPTN

   Plasmid

   #188784

   Purpose

   For mammalian expression of OPTN tagged with mEGFP

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fire Lab C. Elegans Kit

   Plasmid Kit

   #1000000001

   Purpose

   Worm (C. elegans) vector set comprised of lacZ/GFP fusions, exon and intron GFP tagging vectors, RNAi controls, and hairpin sequences for testing the ability of promoters to in vivo trigger RNAi

   Depositor

   Andrew Fire

   Available Since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

6. AAV-∆Hapln1

   Plasmid

   #200778

   Purpose

   Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   cfGFP

   Mutation

   deleted lectican binding domain (aminoacids 40-15…

   Promoter

   Synapsin

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFX-Tom20-ｍCherry

   Plasmid

   #174188

   Purpose

   Express mCherry with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with mitchondria-targeting sequence
   

   Tags

   the mitochondria-targeting sequence of TOM20

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG1186 Lyn11-ECFP-FIREmate

   Plasmid

   #205168

   Purpose

   Expresses Lyn11-ECFP-FIREmate in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   Lyn11-ECFP-FIREmate
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV PHP.eB)

   Viral Prep

   #105530-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CMV

   Tags

   EGFP

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

10. UbcH5c WT pET28a

    Plasmid

    #12643

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Expression

    Bacterial

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. GAPDH-CPG-TV

    Plasmid

    #70148

    Purpose

    a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
    

    Use

    Mouse Targeting and Synthetic Biology

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pArcCreER

    Plasmid

    #46389

    Depositor

    Liqun Luo

    Article

    Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

    Inserts

    Arc (Arc Mouse)
    CreERT2
    

    Use

    Mouse Targeting

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCap-SG5-Apr

    Plasmid

    #227579

    Purpose

    Capture plasmid with apramycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

    Plasmid

    #200641

    Purpose

    Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

    Depositor

    Justin English, Fleur Ferguson

    Article

    Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

    Insert

    miniTurboID-6xGGSG linker-FKBPF36V-2xHA
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-L1PA1CH_blast rescue

    Plasmid

    #69611

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Tags

    blast rescue cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. xABEmax

    Plasmid

    #119813

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. C1(1-29)-miniTurbo-V5_pCDNA3

    Plasmid

    #107174

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBES1-LacOx50-mCh-bla

    Plasmid

    #210260

    Purpose

    Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    mCherry
    

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3 2 kb prom. CD274

    Plasmid

    #107003

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pScaf-1512.1

    Plasmid

    #111402

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-1512
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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