164,868 results

1. pLKO.1.sh.beta-catenin.1248

   Plasmid

   #19761

   Depositor

   William Hahn

   Article

   Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

   Insert

   small hairpin RNA against beta-catenin (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTK91_GFP-MRLC2

   Plasmid

   #46355

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   MRLC2 (MYL12B Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   pBABE

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-EGFP-Cdc42(Q61L)

   Plasmid

   #12600

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (Q61L) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCMV Puro DEST P38KTRDronpa

   Plasmid

   #205763

   Purpose

   Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   P38 KTR Dronpa
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Xiap-Myc

   Plasmid

   #11833

   Depositor

   Guy Salvesen

   Article

   Takahashi et al J Biol Chem. 1998 Apr 3. 273(14):7787-90

   Insert

   XIAP (XIAP Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK290 (mAID-mClover-Hygro)

   Plasmid

   #72828

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Hygro
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN251

   Plasmid

   #188471

   Purpose

   Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   enCas12a-HF (gift from Dr. Keith Joung)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-ZDHHC14

   Plasmid

   #231662

   Purpose

   Expresses human ZDHHC14 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC14 (ZDHHC14 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2/shp53/GFP4

   Plasmid

   #20208

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   shp53/GFP4
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-EGFP-FLAG-PLIN3

    Plasmid

    #161918

    Purpose

    Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Perilipin-3 (PLIN3 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-SIGLEC7-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156773

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SIGLEC7 (SIGLEC7 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDestTol2pA2-U6:gRNA

    Plasmid

    #63157

    Purpose

    Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSGKP-km

    Plasmid

    #117233

    Purpose

    A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

    Plasmid

    #196191

    Purpose

    Tagging human SOX2 gene with P2A-EGFP at the C-terminus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
    

    Use

    Homology repair donor

    Tags

    Expression

    Mutation

    G2913A mutation to replace the guide cutting site…

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO-CreERT2 stuffer v3

    Plasmid

    #158046

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pESF140_HisSpy_DDB1∆B

    Plasmid

    #124213

    Purpose

    Insect cell expression vector for His6, Spy tagged hsDDB1∆B

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    DNA damage-binding protein 1 (DDB1 Human)
    

    Use

    Tags

    His6-Spy

    Expression

    Insect

    Mutation

    internal deletion of BPB domain to aid crystalliz…

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EasyFusion T2A-H2B-miRFP703

    Plasmid

    #113097

    Purpose

    General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-miRFP703
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR1A no ccDB (w48-1)

    Plasmid

    #17398

    Purpose

    Gateway entry 1A vector, no ccDB gene

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF2536

    Plasmid

    #142397

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX6 (SOX6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+ Dkk1-flag

    Plasmid

    #16690

    Depositor

    Xi He

    Article

    Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

    Insert

    Dkk1 (DKK1 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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