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pRSET FLIPPi-30m

Plasmid

#13556

Depositor

Wolf Frommer

Publication

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T22A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T22A in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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LPCX Smad3

Plasmid

#12638

Depositor

Rik Derynck

Publication

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles
pGEX-4T1-14-3-3 gamma GST

Plasmid

#13280

Depositor

Michael Yaffe

Publication

Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

Insert

14-3-3 gamma (Ywhag Rat)

Tags

GST

Expression

Bacterial

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
GFP-siRNA control

Plasmid

#10900

Depositor

Bob Weinberg

Publication

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siGFP

Expression

Mammalian

Available since

Feb. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pUC8 mouse IFN-gamma

Plasmid

#10955

Depositor

Howard Young

Insert

interferon-gamma (Ifng Mouse)

Use

Cloning vector

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmyc-MOV10

Plasmid

#10977

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MOV10 (MOV10 Human)

Tags

myc

Expression

Mammalian

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFLAG/HA-TNRC6B

Plasmid

#10979

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNRC6B (TNRC6B Human)

Tags

FLAG and HA

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSUPER.PKCzeta.RNAi

Plasmid

#10803

Depositor

Alex Toker

Publication

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC zeta RNAi (PRKCZ Human)

Use

RNAi

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
FLAG.PKCzeta.T410E

Plasmid

#10804

Depositor

Alex Toker

Publication

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Activation loop active: T410E (Thr 410 to Glu)

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
1053 pBabe puroL Akt K179M T308A S473A

Plasmid

#9013

Depositor

William Sellers

Publication

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 K179M T308A S473A (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

K179M T308A S473A

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pPS2991

Plasmid

#8931

Depositor

Pamela Silver

Publication

Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NDR2 (STK38L Human)

Tags

FLAG

Expression

Mammalian

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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B-95.ΔA

Bacterial strain

#197933

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Publication

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial resistance

None

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only
pMD2.G-K47Q/R354Q

Plasmid

#207323

Purpose

VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

Depositor

Feng Zhang

Publication

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSV-G (VSIVgp4 Vesicular Stomatitis Virus)

Expression

Mammalian

Mutation

K47Q/R354Q

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-hSyn-Soma-jGCaMP8m (AAV9)

Viral prep

#169257-AAV9

Purpose

Ready-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8m (#169257). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8m plasmid DNA. Syn-driven expression of soma-targeted calcium sensor jGCaMP8m. These AAV preparations are suitable purity for injection into animals.

Depositor

Marianne Fyhn

Publication

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only
pLV-hSyn1-GFP

Plasmid

#177810

Purpose

Encodes a GFP driven by human synapsin I promoter

Depositor

Christopher Grigsby

Publication

Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

Insert

CopGFP inserted behind human synapsin I promoter (SYN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Syn1

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_DA-mut (AAV9)

Viral prep

#140555-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA-mut (#140555). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA-mut plasmid DNA. Synapsin-driven expression of control dopamine sensor GRAB_DA-mut (does not respond to DA). These AAV preparations are suitable purity for injection into animals.

Depositor

Yulong Li

Publication

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Promoter

Syn

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only
Gi2-CASE

Plasmid

#168121

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

Depositor

Gunnar Schulte

Publication

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)

Use

Luciferase

Tags

cpVenus on GNG2

Expression

Mammalian

Mutation

NLuc is inserted at C112/E115 within GNAI2

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-hSyn-Soma-jGCaMP8m

Plasmid

#169257

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Publication

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has service

AAV1, AAV9, and AAV Retrograde

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Publication

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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