167,846 results

1. pBroad-T7RNAP-chlorR

   Plasmid

   #199992

   Purpose

   T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   T7RNAP Circuit
   

   Expression

   Bacterial

   Mutation

   copFnull

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

   Plasmid

   #205317

   Purpose

   All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISYmu1 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-spCas9-N57

   Plasmid

   #169920

   Purpose

   Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   SpCas9-N57
   

   Use

   CRISPR

   Mutation

   N57 is the PAI domain of SB100X

   Promoter

   CAG

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-NLS-actin-R62D

   Plasmid

   #118381

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Mutation

   NLS and R62D

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmEGFP_a_tubulin_C1

   Plasmid

   #21039

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Tags

   pmEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBA560

   Plasmid

   #186236

   Purpose

   pTet-eLbuCas13a crRNA entry vector

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5'Entry-lck-5.5kb

   Plasmid

   #58890

   Purpose

   provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

   Depositor

   Jeffrey Yoder

   Insert

   zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
   

   Use

   Gateway 5' entry clone

   Mutation

   mutated single nucleotide (T-->C, 13th nucleot…

   Promoter

   lymphocyte-specific protein tyrosine kinase (lck)…

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-V5-Puro

   Plasmid

   #207730

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pMK232 (CMV-OsTIR1-PURO)

   Plasmid

   #72834

   Purpose

   Expresses OsTIR1 under the control of the CMV promoter

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   AAVS1-CMV-OsTIR1-PURO-AAVS1
   

   Expression

   Mammalian

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. V5-BS2-NES (Cytosol)

    Plasmid

    #220341

    Purpose

    Mammalian expression vector BS2 in cytoplasm

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Tags

    NES (Nuclear exclusion sequence) and V5 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3.3_c-MYC

    Plasmid

    #26818

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-c-MYC-3'UTR (MYC Human)
    

    Expression

    Mammalian

    Mutation

    Sequence begins from second ATG; Synthetic UTR se…

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEGFP-N1-IL6

    Plasmid

    #111933

    Purpose

    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Interleukin 6 (IL6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.23-GW

    Plasmid

    #60323

    Purpose

    This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pL

    Plasmid

    #196286

    Purpose

    35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV L antigenome encoding codon-optimized L (RdRp)
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pScaf-5544.1

    Plasmid

    #111405

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-GH'
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGW-TurboID-GOI

    Plasmid

    #209380

    Purpose

    Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMAZ-SK

    Plasmid

    #73962

    Purpose

    self-killing gRNA plasmid pMAZ-SK

    Depositor

    Alex Nielsen

    Article

    Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

    Insert

    gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-KASH2

    Plasmid

    #187017

    Purpose

    Inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EMMA toolkit

    Plasmid Kit

    #1000000119

    Purpose

    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production

    Depositor

    Yizhi Cai

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pEGFP-N1-1x

    Plasmid

    #172281

    Purpose

    Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

    Depositor

    Antony K Chen

    Article

    Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

    Insert

    EGFP-N1-1x
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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