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c-myc promoter (TBE 1/2 - wt)
(Plasmid #16595)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16595 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBV-Luc
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 4867
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-myc promoter (TBE 1/2 wt)
  • Alt name
    MYC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

To construct pTBE1/2 plasmid, we used polymerase chain reaction (PCR) primers (5'-CTAGCTAGCCTAGCACCTTTGATTTCTCCC-3' and 5'-CGTGATATCCGCTTTGATCAAGAGTCCCAG-3') to amplify nt -576 to -1162 of the c-MYC promoter region. The PCR product was cloned into pBV-Luc.

TBE1 (Tcf-4 binding elements) wt sequence cctttgatt; TBE2 wt sequence gctttgatc.

See Author's Map and article for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    c-myc promoter (TBE 1/2 - wt) was a gift from Bert Vogelstein (Addgene plasmid # 16595 ; http://n2t.net/addgene:16595 ; RRID:Addgene_16595)
  • For your References section:

    Identification of c-MYC as a target of the APC pathway. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW. Science. 1998 Sep 4. 281(5382):1509-12. 10.1126/science.281.5382.1509 PubMed 9727977