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c-myc promoter (TBE 1m/2m - mut)
(Plasmid #16596)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 16596 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 4867
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    c-myc promoter (TBE 1m/2m mut)
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Tcf-4 binding elements (TBEs) mutated
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

To construct pTBE1m/2m, we used a mutated TBE1 primer (5'-CTAGCTAGCACTGGTGCATCTCCCAAACCCGGCAGCCCG-3') and a mutated TBE2 primer (5'-CTGGATATCACTGGTGCATCCCAGGGAGAGTGGAGGAAAG-3'), and subcloned the products into pBV-Luc.

See Author's Map and article for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    c-myc promoter (TBE 1m/2m - mut) was a gift from Bert Vogelstein (Addgene plasmid # 16596 ; ; RRID:Addgene_16596)
  • For your References section:

    Identification of c-MYC as a target of the APC pathway. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW. Science. 1998 Sep 4. 281(5382):1509-12. 10.1126/science.281.5382.1509 PubMed 9727977