c-myc promoter (4x TBE2 - wt)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16599||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namec-myc promoter (4xTBE2 wt)
SpeciesH. sapiens (human)
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Tcf-4 binding elements (TBEs). To construct the four tandem repeats of TBE2, we dimerized oligonucleotide cassettes containing two copies of the site and cloned the products into pBV-Luc (5'-CTAGCGGACTCTTGATCAAAGGACTCTTGATCAAAG-3' and 5'-CTAGCTTTGATCAAGAGTCCTTTGATCAAGAGTCCG-3')
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:c-myc promoter (4x TBE2 - wt) was a gift from Bert Vogelstein (Addgene plasmid # 16599 ; http://n2t.net/addgene:16599 ; RRID:Addgene_16599)
For your References section:Identification of c-MYC as a target of the APC pathway. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW. Science. 1998 Sep 4. 281(5382):1509-12. 10.1126/science.281.5382.1509 PubMed 9727977
Map uploaded by the depositor.