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GST TopBP1 (aa 32-1522) His
(Plasmid #20375)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 20375 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name
    topoisomerase II beta binding protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Starts at aa32
  • Entrez Gene
    TOPBP1 (a.k.a. Dpb11, TOP2BP1)
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His.

NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST TopBP1 (aa 32-1522) His was a gift from Aziz Sancar (Addgene plasmid # 20375 ; ; RRID:Addgene_20375)
  • For your References section:

    Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA. Choi JH, Lindsey-Boltz LA, Sancar A. Nucleic Acids Res. 2009 Jan 12. ():. 10.1093/nar/gkn1075 PubMed 19139065