GST TopBP1 (aa 32-1522) His
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20375||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeBacterial Expression
Growth in Bacteria
Alt nametopoisomerase II beta binding protein 1
SpeciesH. sapiens (human)
Insert Size (bp)4566
MutationStarts at aa32
Entrez GeneTOPBP1 (a.k.a. Dpb11, TOP2BP1)
/ Fusion Protein
- 6xHis (C terminal on insert)
- Cloning method Restriction Enzyme
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His.
NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GST TopBP1 (aa 32-1522) His was a gift from Aziz Sancar (Addgene plasmid # 20375 ; http://n2t.net/addgene:20375 ; RRID:Addgene_20375)
For your References section:Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA. Choi JH, Lindsey-Boltz LA, Sancar A. Nucleic Acids Res. 2009 Jan 12. ():. 10.1093/nar/gkn1075 PubMed 19139065
Map uploaded by the depositor.