We narrowed to 10,190 results for: yeast

1. pCfB3052(gRNA X-4, XI-3, XII-5)

   Plasmid

   #73294

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC5GGv2

   Plasmid

   #188604

   Purpose

   pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. 416Gal-FUS-R521C-YFP

   Plasmid

   #29622

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   R521C

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. TRP1MX6-ins4

   Plasmid

   #195041

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   TRP1
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGE2.0-i

   Plasmid

   #194639

   Purpose

   Empty pAGE2.0 plasmid with pBAD inducible promoter

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. kanMX6-ins6

   Plasmid

   #195043

   Purpose

   pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB3051(gRNA X-3 XI-2 XII-2)

   Plasmid

   #73293

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. 416Gal-FUS-R521G-YFP

   Plasmid

   #29623

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   YFP

   Expression

   Yeast

   Mutation

   R521G

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

9. 416Gal-SV40NLS-FUS-YFP

   Plasmid

   #29595

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   NLS-FUS (FUS Human)
   

   Tags

   SV40 NLS and YFP

   Expression

   Yeast

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

10. his3MX6-ins3

    Plasmid

    #195040

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    His5+
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. G31, Cdc20-AID tagging

    Plasmid

    #130269

    Purpose

    Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

    Depositor

    Matthias Heinemann

    Article

    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

    Insert

    Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
    

    Tags

    AID(71-114)

    Expression

    Yeast

    Promoter

    Native promoter

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p426_Cas9_gRNA-ARS106a

    Plasmid

    #87382

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS106a sequence ATACGGTCAGGGTAGCGCCC in yeast chromosome 1.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS106a
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 426Gal-FUS-RRM Mutant-YFP

    Plasmid

    #29608

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS RRM mutant (FUS Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    F305L, F341L, F359L and F368L

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pUDE710

    Plasmid

    #103020

    Purpose

    expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS-mTagRFP-TUBA1B

    Plasmid

    #62850

    Purpose

    Donor vector for gene editing at human TUBA1B locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    mTagRFP-TUBA1B (TUBA1B Synthetic, Human)
    

    Use

    Yeast-e.coli shuttle vector

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBSWHhc-p53

    Plasmid

    #133903

    Purpose

    Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    p53 (TP53 Mouse)
    

    Expression

    Yeast

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p426_Cas9_gRNA-ARS1114a

    Plasmid

    #87397

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1114a sequence CTTGTGAAACAAATAATTGG in yeast chromosome 11.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1114a
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p426_Cas9_gRNA-ARS308a

    Plasmid

    #87384

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS308a sequence CACTTGTCAAACAGAATATA in yeast chromosome 3

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS308a
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pH-eCFP

    Plasmid

    #176555

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pL-eCFP

    Plasmid

    #176556

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of leucine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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