Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 15,460 results for: pET

Showing: 9861 - 9880 of 15460 results

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TTR C10S S85C F87E

Plasmid

#238074

Purpose

Bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S S85C F87E

Promoter

T7

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF4x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235316

Purpose

Lentiviral expression of mRuby2 with 5' microRNA target site only (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP-FMRP-bGH

Plasmid

#235265

Purpose

ComMAND EGFP-FMRP base gene

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF6x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235323

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF6x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235315

Purpose

Lentiviral expression of mRuby2 with 5' microRNA target site only (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF6x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235321

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235313

Purpose

Lentiviral expression of mRuby2 with 3' microRNA target site only (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235319

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235314

Purpose

Lentiviral expression of mRuby2 with 3' microRNA target site only (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF4x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235322

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF4x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235324

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGH020 sgControl (GEA)

Plasmid

#228193

Purpose

Safe targeting sgRNA

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Safe targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGH020 sgControl (ACOCB)

Plasmid

#228194

Purpose

Safe targeting sgRNA

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Safe targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdnCas3-CDA1

Plasmid

#229535

Purpose

pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

dnCas3
Cytidine deaminase
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) C90A, T172C

Plasmid

#217983

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

C90A, T172C

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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Hygro-P2A-HA-FKBP-WAPL

Plasmid

#217666

Purpose

Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

WapI (WAPL Human)

Tags

dTag (FKBP36V protein)

Expression

Bacterial and Mammalian

Promoter

Lac

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) T160C

Plasmid

#220233

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

T160C

Promoter

T7

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(85-160)

Plasmid

#217984

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Promoter

T7

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-100)

Plasmid

#217978

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Promoter

T7

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9861 - 9880 of 15460 results

Results per page:

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