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pT7-EGFP-C1-HsNot2-W507E_S

Plasmid

#147511

Purpose

Mammalian Expression of HsNot2-W507E

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-W507E (CNOT2 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-41P-HsNot2_344-540_M

Plasmid

#146946

Purpose

Bacterial Expression of HsNot2_344-540

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_344-540 (CNOT2 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2_305-540_K

Plasmid

#146793

Purpose

Mammalian Expression of HsNot2_305-540

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_305-540 (CNOT2 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2_344-540_K

Plasmid

#146794

Purpose

Mammalian Expression of HsNot2_344-540

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_344-540 (CNOT2 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrUPF1_F

Plasmid

#146281

Purpose

Bacterial Expression of DrUPF1

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF1 (upf1 Zebrafish)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic-PTC62-deltaIntron3_D

Plasmid

#146146

Purpose

Mammalian Expression of tubgenomic-PTC62-delIntron3

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic-PTC62-delIntron3

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 F355R

Plasmid

#195209

Purpose

Mammalian expression vector for GFP tagged NHERF1. Ezrin interaction-deficient mutant

Depositor

Anthony Bretscher, David Bryant

Publication

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

F355R

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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CIT1-2EGFP-ter-URA3

Plasmid

#184767

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CIT1 (CIT1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AFG3-2EGFP-ter-URA3

Plasmid

#184770

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

AFG3 (AFG3 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_PM

Plasmid

#182813

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_cyto

Plasmid

#182814

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_ER

Plasmid

#182815

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_PM

Plasmid

#182816

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_ER

Plasmid

#182812

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pENT-L3-eBra-L4

Plasmid

#186368

Purpose

Entry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Brachury notochord enhancer

Use

Protein expression

Promoter

T7 promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L3-Titf-L4

Plasmid

#186367

Purpose

Entry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Titf endoderm enhancer

Use

Protein expression

Promoter

T7 promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L3-pSnail-L5

Plasmid

#186365

Purpose

Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Snail regulatory sequence

Use

Protein expression

Promoter

T7 promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-HsGIGYF1_1-671_AI

Plasmid

#148958

Purpose

Mammalian Expression of HsGIGYF1_1-671

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1_1-671 (GIGYF1 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1_1-671_AI

Plasmid

#148943

Purpose

Mammalian Expression of HsGIGYF1_1-671

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1_1-671 (GIGYF1 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1-F306AF312A_AI

Plasmid

#148945

Purpose

Mammalian Expression of HsGIGYF1-F306AF312A

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1-F306AF312A (GIGYF1 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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