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CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
Iverson SV, Haddock TL, Beal J, Densmore DM
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
65980J23100_ABMoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23100:B]
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65981J23100_EBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [E:J23100:B]
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65982J23100_FBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [F:J23100:B]
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65983J23100_GBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]
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65984J23102_ABMoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23102:B]
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65985J23102_EBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [E:J23102:B]
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65986J23102_FBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [F:J23102:B]
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65987J23102_GBMoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23102:B]
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65988J23103_ABMoClo Basic Part: Constitutive promoter - Anderson series - low strength [A:J23103:B]
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65989J23103_EBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [E:J23103:B]
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65990J23103_FBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [F:J23103:B]
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65991J23103_GBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]
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65992J23106_ABMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [A:J23106:B]
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65993J23106_EBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [E:J23106:B]
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65994J23106_FBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [F:J23106:B]
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65995J23106_GBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23106:B]
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65996J23107_ABMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [A:J23107:B]
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65997J23107_EBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [E:J23107:B]
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65998J23107_FBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [F:J23107:B]
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65999J23107_GBMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]
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66000J23116_ABMoClo Basic Part: Constitutive promoter - Anderson series - low strength [A:J23116:B]
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66001J23116_EBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [E:J23116:B]
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66002J23116_FBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [F:J23116:B]
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66003J23116_GBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]
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66004R0010 (pLacI)_ABMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]
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66005R0010 (pLacI)_EBMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [E:R0010:B]
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66006R0010 (pLacI)_FBMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [F:R0010:B]
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66007R0010 (pLacI)_GBMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [G:R0010:B]
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66008R0040 (pTet)_ABMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B]
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66009R0040 (pTet)_EBMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B]
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66010R0040 (pTet)_FBMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B]
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66011R0040 (pTet)_GBMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B]
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66012R0063 (pLuxR-pR)_ABMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B]
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66013R0063 (pLuxR-pR)_EBMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [E:R0063:B]
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66014R0063 (pLuxR-pR)_FBMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B]
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66015R0063 (pLuxR-pR)_GBMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [G:R0063:B]
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66016I13453 (pBAD)_ABMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [A:I13453:B]
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66017I13453 (pBAD)_EBMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [E:I13453:B]
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66018I13453 (pBAD)_FBMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B]
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66019I13453 (pBAD)_GBMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B]
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66020B0032m_BCMoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]
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66021B0033m_BCMoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C]
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66022B0034m_BCMoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C]
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66023BCD12_BCMoClo Basic Part: RBS - BiCistronic Design, medium strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD12:C]
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66024BCD2_BCMoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C]
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66025BCD8_BCMoClo Basic Part: RBS - BiCistronic Design, low strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD8:C]
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66026C0012m (lacI)_CDMoClo Basic Part: CDS - Controller protein, lacI repressor (in concert with CAP, represses pLacI, R0010). Modified to fix illegal site. [C:C0012m:D]
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66027C0040 (tetR)_CDMoClo Basic Part: CDS - Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) [C:C0040:D]
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66028C0062 (luxR)_CDMoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D]
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66029C0080 (araC)_CDMoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]
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66030cre_CDMoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D]
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66031E0030 (YFP)_CDMoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]
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66032E0040m (GFP)_CDMoClo Basic Part: CDS - Fluorescent protein. Green. Modified from Bba_E0040 to fix illegal site. [C:E0040m:D]
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66033E1010m (RFP)_CDMoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]
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66034eBFP2 (eBFP2)_CDMoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]
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66035B0015_DEMoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:E]
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66036B0015_DFMoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:F]
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66037B0015_DGMoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:G]
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66038B0015_DHMoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:H]
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66039DVA_ABMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:B]
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66040DVA_AEMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:E]
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66041DVA_AFMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:F]
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66042DVA_AGMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:G]
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66043DVA_AHMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:H]
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66044DVA_BCMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [B:LacZa:C]
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66045DVA_CDMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [C:LacZa:D]
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66046DVA_DEMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:E]
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66047DVA_DFMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:F]
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66048DVA_DGMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:G]
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66049DVA_DHMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H]
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66050DVA_EBMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:B]
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66051DVA_EFMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:F]
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66052DVA_EGMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:G]
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66053DVA_EHMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:H]
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66054DVA_FBMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [F:LacZa:B]
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66055DVA_GBMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [G:LacZa:B]
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66056DVAMoClo Destination Vector: Empty vector for creating new DVA. Cut with SpeI and insert amplified LacZa (based on pSB1A2)
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66057pJ02B2Gm_AEMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]
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66058pJ02B2Gm_EFMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]
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66059pJ02B2Rm_AEMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]
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66060pJ02B2Rm_EFMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]
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66061pJ02B2Gm:Rm_AFMoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]
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66062pJ02B2Rm:Gm_AFMoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]
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66063pJ02B2Gm_AEMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]
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66064pJ02B2Gm_EFMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]
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66065pJ02B2Rm_AEMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]
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66066pJ02B2Rm_EFMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]
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66067DVK_AEMoClo Destination Vector: (based on pSB1K3) [A:LacZa:E]
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66068DVK_AFMoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]
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66069DVK_EFMoClo Destination Vector: (based on pSB1K3) [E:LacZa:F]
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66070DVK_FGMoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]
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66071DVK_GHMoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]
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66072DVKMoClo Destination Vector: Empty vector for creating new DVK. Cut with SpeI and insert amplified LacZa (based on pSB1K3)
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