E0030 (YFP)_CD
(Plasmid
#66031)
-
PurposeMoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]
-
Depositing Lab
-
Depositing OrganizationBoston University Main Campus
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 66031-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 66031-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneDVA
-
Vector typeSynthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameFluorescent reporter - YFP
-
Alt nameBasic Part - CDS
-
SpeciesSynthetic
Cloning Information
- Cloning method Unknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DNA (Catalog # 66031-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 66031-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
E0030 (YFP)_CD was a gift from Douglas Densmore (Addgene plasmid # 66031 ; http://n2t.net/addgene:66031 ; RRID:Addgene_66031) -
For your References section:
CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. Iverson SV, Haddock TL, Beal J, Densmore DM. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. 10.1021/acssynbio.5b00124 PubMed 26479688
