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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 21,445 results for: cas9

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Showing: 9961 - 9980 of 21445 results

  2. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Publication
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107999-plasmid-map-sequence-id-217151

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  4. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    68710-plasmid-map-sequence-id-123992

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  8. pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

    Plasmid
    #228722
    Purpose
    Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228722-plasmid-map-sequence-id-454017

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  9. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid
    #228723
    Purpose
    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2 nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228723-plasmid-map-sequence-id-454010

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  10. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid
    #228724
    Purpose
    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228724-plasmid-map-sequence-id-454004

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  11. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Publication
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  12. pMLS716

    Plasmid
    #188888
    Purpose
    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre
    Depositor
    Erik Jorgensen
    Publication
    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.
    Insert
    floxed Cbr-unc-119 marker and Phsp16.41::Cre
    Expression
    Worm
    Mutation
    C. elegans codom optimization and intron addition
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188888-plasmid-map-sequence-id-370784

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Showing: 9961 - 9980 of 21445 results