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pCherryGFP-CoV2-WT

Plasmid

#177618

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF1

Plasmid

#17997

Depositor

Robert Tjian

Article

Ruppert et al Nature. 1993 Mar 11. 362(6416):175-9.

Insert

hTAF1 (TAF1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_I770A_6xH

Plasmid

#185711

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed I770 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-syn-fRGECO1

Plasmid

#125243

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO1

Expression

Mammalian

Mutation

D323A

Promoter

synapsin

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI3M

Plasmid

#249061

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI3M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEY11

Plasmid

#191017

Purpose

Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

Ultrapanneuronal

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_ABCE1-Halo

Plasmid

#175335

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

ABCE1 (ABCE1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL7086

Plasmid

#167250

Purpose

orthogonal Mango reporter output for cell-free expression

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pAKSIRV-Mango

Use

Synthetic Biology

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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CMV::TVA/tRFP

Plasmid

#164486

Purpose

Dual CMV promoter plasmid to express TVA and TurboRFP

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Inserts

TVA (CD320 Chicken)
TurboRFP

Expression

Mammalian

Promoter

sCMV and sCMV IE94

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A/T1462A)

Plasmid

#14131

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A/T1462A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A/T1462A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
RA-Sec61β

Plasmid

#153978

Purpose

Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

Sec61 Transolocon Subunit Beta (SEC61B Human)

Tags

pAcGFP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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gRNA-ura-HYB

Plasmid

#64330

Purpose

ura3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of ura3 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-DDX3X

Plasmid

#70648

Purpose

Expresses WT DDX3X in mammalian cells under blasticidin selection.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

DDX3X (DDX3X Human)

Use

Retroviral

Tags

3x FLAG

Expression

Mammalian

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pFastBac-His-MBP-SCov2-nsp12

Plasmid

#154759

Purpose

Expression of SARS-CoV-2 nsp12

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Insect

Promoter

Polyhedron

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGDP3 aph(3'')-Ia

Plasmid

#112879

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139484

Purpose

PCR template for mEGFP gene knock-in donor vector

Depositor

Kazuhiro Aoki

Article

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

mEGFP-loxP-P2A-dTKneo-pA-loxP

Tags

mEGFP

Expression

Mammalian

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP_mEGFP-GluA1c

Plasmid

#114136

Purpose

Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mEGFP tagged GluA1 C-terminus

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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SNAP-Dcp2

Plasmid

#153975

Purpose

SNAP-tag polypeptide fused to mRNA decapping enzyme 2

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA Decapping enzyme 2 (DCP2 Human)

Tags

SNAP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/Δp120

Plasmid

#45770

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Δp120 (CDH1 Human)

Expression

Mammalian

Mutation

Amino acids 762-764 converted from EED to AAA to …

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCherryGFP-Inframe-noFSE

Plasmid

#177619

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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