We narrowed to 50,536 results for: kan

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pRha-ABE7.10-SpCas9-NG

Plasmid

#170663

Purpose

Expresses the base editor ABE7.10-SpCas9-NG in bacterial cells

Depositor

David Liu

Publication

Krishnamurthy et al Nucleic Acids Res. 2021 Oct 11;49(18):10

Insert

ABE7.10-SpCas9-NG

Tags

8xHIS and BP-NLS

Expression

Bacterial

Promoter

pRhaBAD

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMC_MS2_DNA_VLP

Plasmid

#179156

Purpose

Expression of maturation protein and his-tagged coat protein dimer from MS2 phage

Depositor

Paul Freemont

Publication

Crone et al GEN Biotechnol. 2022 Dec 1;1(6):496-503.

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available since

Sept. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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FRB-mCherry-Giantin

Plasmid

#186575

Purpose

Expression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)

Depositor

Stephen Royle

Publication

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Giantin (GOLGB1 Human)

Tags

FRB-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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piGECInano-N1

Plasmid

#186190

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iGECInano

Expression

Mammalian

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP

Plasmid

#183934

Purpose

PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

Depositor

Karsten Rippe

Publication

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tdPCP-GFP

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Calnexin-HT-mGold

Plasmid

#183987

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.

Depositor

Pablo Rivera-Fuentes

Publication

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Calnexin (CANX Human)

Tags

HaloTag, mGold

Expression

Mammalian

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-SpRY-His

Plasmid

#179320

Purpose

Plasmid for bacterial expression and purification of SpRY-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Publication

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpRY

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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hEGFR-mEGFP

Plasmid

#182878

Purpose

Mammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFP

Depositor

Salvatore Chiantia

Publication

Tzoneva et al Biomolecules. 2020 May 22;10(5). pii: bi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hEGFR-mEGFP (EGFR Human)

Expression

Mammalian

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-mSA*

Plasmid

#182952

Purpose

Express SpCas9-mSA*

Depositor

Wen Xue

Publication

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9-mSA*

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_EIF4E_eiF4E

Plasmid

#178474

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Publication

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EIF4E_eiF4E (EIF4E Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Kras

Plasmid

#180571

Purpose

ddGFP sensor of Kras

Depositor

Won Do Heo

Publication

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ddGFP sensor of Kras

Expression

Mammalian

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEB2_mScarletI_mVenusNB-MrkH

Plasmid

#182291

Purpose

expresses c-di-GMP biosensor in E. coli

Depositor

Howard Berg

Publication

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkH

Tags

mVenusNB internal fusion to MrkH

Expression

Bacterial

Promoter

proC

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFX-EGFP-Rab5

Plasmid

#174454

Purpose

Express EGFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFX-mCherry-Rab11

Plasmid

#174461

Purpose

Express mCherry with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry with recycling endosome-targeting sequence

Tags

small GTPase (Rab11)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFX-mCherry-Rab7

Plasmid

#174458

Purpose

Express mCherry with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -0

Plasmid

#180007

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AF1521-c003

Plasmid

#173111

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Tags

Nanoluc

Expression

Bacterial

Mutation

KH-M1-L192

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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mILK-EFGP

Plasmid

#176896

Purpose

mouse ILK cloned into pEGFP-N1

Depositor

Sara Wickström

Publication

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PtxS1-c003

Plasmid

#173076

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PtxS1

Expression

Bacterial

Mutation

D35-I221

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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