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Showing: 81 - 100 of 150 results

  1. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid
    #135668
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgCR8
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135668-plasmid-map-sequence-id-325838

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  2. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid
    #135669
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgPten
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135669-plasmid-map-sequence-id-325841

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  3. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Pten_shRNAmir

    Plasmid
    #178044
    Purpose
    CAG tdGFP Pten shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Pten_1523 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178044-plasmid-map-sequence-id-352916

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  4. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Renilla_shRNAmir

    Plasmid
    #178046
    Purpose
    CAG tdGFP Renilla shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Renilla_713 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178046-plasmid-map-sequence-id-352920

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  5. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir

    Plasmid
    #178045
    Purpose
    CAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Luciferase_1309 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178045-plasmid-map-sequence-id-352919

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  6. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_3

    Plasmid
    #178049
    Purpose
    CAG tdGFP Lrig1 shRNAmir-3 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178049-plasmid-map-sequence-id-352988

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  7. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2

    Plasmid
    #178048
    Purpose
    CAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178048-plasmid-map-sequence-id-352985

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  8. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

    Plasmid
    #178047
    Purpose
    CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178047-plasmid-map-sequence-id-352935

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  9. PB CAG-F-intron-L-F-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #178433
    Purpose
    CAG tdGFP FLP/FRT cre/lox "NOT" reporter piggyBac transposon (bpA SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox; Flp/frt, piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178433-plasmid-map-sequence-id-350247

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  10. PB CAG-F-bpA-SV40pA-F-L-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #178432
    Purpose
    CAG tdGFP FLP/FRT cre/lox "AND" reporter piggyBac transposon (bpA SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox; Flp/frt, piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178432-plasmid-map-sequence-id-350248

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  12. pENTR-Flippase-myc

    Plasmid
    #139548
    Purpose
    Flippase-myc fusion ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Fleppase-myc
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139548-plasmid-map-sequence-id-274696

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  13. pCALNL-tdTomato

    Plasmid
    #194205
    Purpose
    Cre/LoxP-dependent expression of a tdTomato. Expression of Cre excises a STOP codon before tdTomato, facilitating fluorescence expression of tdTomato.
    Depositor
    Angelique Bordey
    Insert
    tdTomato
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194205-plasmid-map-sequence-id-382464

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  17. pTOPO-col10a1-KI-donor

    Plasmid
    #184874
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Collagen10a1 5' homology arm
    Collagen10a1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    eGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    184874-plasmid-map-sequence-id-363155

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  19. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

    Plasmid
    #229814
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    loxp-EGFP-stop-lox
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229814-plasmid-map-sequence-id-460474

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  20. pJET-col2a1a-KI-donor

    Plasmid
    #184876
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Collagen2a1a 5' homology arm
    Collagen2a1a 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    184876-plasmid-map-sequence-id-362292

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Showing: 81 - 100 of 150 results