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p8270 LentiCRISPR v2 hygro sgSIGIRR-3

Plasmid

#193979

Purpose

Expression of spCas9 and sgRNA targeting SIGIRR

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)

Use

Lentiviral

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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eSrtA-Δ24-ASN

Plasmid

#221849

Purpose

Expresses delta 24 alpha synuclein as 6XHis-SUMO-eSrtA-LPNTG-Δ24-ASN fusion protein

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Mutation

No

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Plasmid

#222987

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL390

Plasmid

#219500

Purpose

Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT

Tags

SV40-NLS

Expression

Yeast

Promoter

GAL1-GAL10

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL391

Plasmid

#219501

Purpose

Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1

Expression

Yeast

Promoter

GAL7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pKH160_FLAG-APEX2-YIPF4 LIR mut

Plasmid

#214979

Purpose

Flag-APEX2 tagged LIR mutant YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2_YIPF4_mut (YIPF4 Human)

Use

Lentiviral

Mutation

LIR mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH145_mCherry-YIPF4 LIR mut

Plasmid

#214973

Purpose

mCherry tagged LIR mutant YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mCherry-YIPF4_mut (YIPF4 Human)

Use

Lentiviral

Mutation

LIR mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-FL

Plasmid

#215839

Purpose

Expression of FLAG/HA tagged firefly luciferase (FL)

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA tagged firefly luciferase (FL)

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK1-CXCR4-MM4

Plasmid

#215846

Purpose

Expression of CXCR4 target with mismatch (MM) 4_

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

CXCR4 target with mismatch (MM) 4x

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaTW-R1

Plasmid

#210455

Purpose

Express waaT and waaW from E. coli R1 under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaT and waaW

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaTW-R4

Plasmid

#210456

Purpose

Express waaT and waaW from E. coli R4 under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaT and waaW

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh10.1 GFP

Plasmid

#209102

Purpose

GFP expressing scramble of shRNA targeting Cdh10

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh10.1

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCdh11.1 GFP

Plasmid

#209103

Purpose

GFP expressing shRNA targeting Cdh11

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCdh11.1

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh11.1 GFP

Plasmid

#209104

Purpose

GFP expressing scramble of shRNA targeting Cdh11

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh11.1

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCtnnd2.2-GFP

Plasmid

#209098

Purpose

GFP expressing scramble of shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.2

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRAB14-HA

Plasmid

#214165

Purpose

Transient expression of RAB14

Depositor

Nan Yan

Article

Tu et al Nat Commun. 2022 Nov 15;13(1):6977. doi:

Insert

RAB14 (Rab14 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-scrCtnnd2.1-mCherry-CAAX

Plasmid

#209108

Purpose

Membrane tethered mCherry scramble of shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.1

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-shCtnnd2.1-mCherry-CAAX

Plasmid

#209107

Purpose

Membrane tethered mCherry shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.1

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCdh20.1 GFP

Plasmid

#209105

Purpose

GFP expressing shRNA targeting Cdh20

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCdh20.1

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCdh10.1 GFP

Plasmid

#209101

Purpose

GFP expressing shRNA targeting Cdh10

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCdh10.1

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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