We narrowed to 1,086 results for: gibson assembly

1. pmScarlet-I_I3

   Plasmid

   #239341

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet-I in mammalian cells. Assembles into nanocages tagged with 60 FPs.

   Depositor

   Torsten Wittmann

   Article

   Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

   Insert

   I3-01
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmStayGold(E138D)_I3

   Plasmid

   #239338

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold(E138D) in mammalian cells. Assembles into nanocages tagged with 60 FPs.

   Depositor

   Torsten Wittmann

   Article

   Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

   Insert

   I3-01
   

   Tags

   mStayGold(E138D)

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmRuby3_I3

   Plasmid

   #239344

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with mRuby3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

   Depositor

   Torsten Wittmann

   Article

   Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

   Insert

   I3-01
   

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. 4G-Cloning Plasmid Kit

   Plasmid Kit

   #1000000252

   Purpose

   The 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.

   Depositor

   Stephan Gruber

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

5. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

   Plasmid

   #181792

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDOC-GG

   Plasmid

   #149377

   Purpose

   Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

   Depositor

   Mark Pallen

   Article

   Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

   Inserts

   Fragment containing a kanamycin resistance cassette
   Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
   Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
   Fragment containing an I-SceI recognition sequence
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY2ShHELIX_sgRNA_entry (CJT30)

   Plasmid

   #181781

   Purpose

   Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Expression

   Bacterial

   Mutation

   Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro

   Plasmid

   #173230

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro

   Plasmid

   #173231

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid

    #181791

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

    Plasmid

    #181789

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

    Plasmid

    #181790

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-MTS-ccdb-DddA(G1333C)-UGI-Puro

    Plasmid

    #173228

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-MTS-ccdb-DddA(G1333N)-UGI-Puro

    Plasmid

    #173229

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid

    #181788

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid

    #181787

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKG2387_pPV2-mRuby2-P2A-PuroR

    Plasmid

    #239696

    Purpose

    BsaI Golden Gate assembly plasmid encoding the mRuby2-P2A-PuroR CDS

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2-P2A-PuroR
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmEmerald_mEmerald_I3

    Plasmid

    #231681

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with two tandem mEmeralds in mammalian cells. Assembles into nanocages tagged with 120 mEmerald FPs.

    Depositor

    Torsten Wittmann

    Article

    Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

    Insert

    I3-01
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmEmerald_I3

    Plasmid

    #231680

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with one mEmerald in mammalian cells. Assembles into nanocages tagged with 60 mEmerald FPs.

    Depositor

    Torsten Wittmann

    Article

    Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

    Insert

    I3-01
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro

    Plasmid

    #173232

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge