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Plasmid#136282PurposeExpresses a guide RNA targeting HEK3 siteDepositorInsertsgRNA-HEK3
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-Flag-YAP2
Plasmid#27472DepositorInsertYes-kinase associated protein (YAP1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
ManII-SBP-mCherry_puromycin
Plasmid#65261PurposeManII reporter only (no hook) (RUSH system)DepositorInserttruncated Mannosidae II
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgMARK2
Plasmid#138689PurposeExpresses a human MARK2-targeting sgRNA and Cas9DepositorInsertsgMARK2 human (MARK2 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgMARK3
Plasmid#138690PurposeExpresses a human MARK3-targeting sgRNA and Cas9DepositorInsertsgMARK3 human (MARK3 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mMcl-1
Plasmid#32980DepositorInsertMcl-1 (Mcl1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shPRDM14-1
Plasmid#193694PurposeConstitutive lentiviral expression of PRDM14 shRNADepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shPRDM14-2
Plasmid#193695PurposeConstitutive lentiviral expression of PRDM14 shRNADepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shPRDM14-3
Plasmid#193696PurposeConstitutive lentiviral expression of PRDM14 shRNADepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shPRDM14-4
Plasmid#193697PurposeConstitutive lentiviral expression of PRDM14 shRNADepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shPRDM14-5
Plasmid#193698PurposeConstitutive lentiviral expression of PRDM14 shRNADepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-NeuroD1
Plasmid#114278PurposeTET-inducible expression of the NeuroD1 proteinDepositorInsertNeuroD1 (Neurod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3PA-Puro-iTk
Plasmid#109067Purposeinserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selectionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEFSAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
hgRNA-A21_pLKO-Puro
Plasmid#100570PurposehgRNA-A21 in a lentiviral backbone with puromycin resistanceDepositorInserthgRNA-A21
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1-HA-Puro
Plasmid#193767PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1-HA (CDT1 Human)
UseLentiviralTagsHAExpressionMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
UseTagsGFP with 2A protease cleavage site between H2B an…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-StrepTag II-PuroR
Plasmid#80967PurposeCRISPaint gene taggingDepositorInsertStrepTag II
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-PGKnVA
Plasmid#24589DepositorInsertGateway(TM) cassette
UseLentiviralTagsVAExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgULK1-1
Plasmid#109004PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertULK1-sgRNA #1 (ULK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 C334S
Plasmid#40983DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationC334SPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only