We narrowed to 27,608 results for: cas9

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pJMP1356

Plasmid

#119276

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDD143

Plasmid

#119880

Purpose

Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

Depositor

Tae Seok Moon

Publication

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_dCas9sth1

Expression

Bacterial

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

Plasmid

#208957

Purpose

A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Publication

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry Codon 59 sgRNA

Plasmid

#109431

Purpose

MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

Depositor

Reuben Harris

Publication

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry codon 59 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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P3-Lenti-dCas9-Tet1IM-GFP

Plasmid

#190730

Purpose

Lentiviral construct to express spdCas9 fused to the de-activated catalytic domain of Tet1 (Tet1IM) and GFP.

Depositor

Maja Jagodic

Publication

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

SV40 NLS and T2A-EGFP

Expression

Mammalian

Promoter

UbC

Available since

Oct. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pJS15A-TCas9-adhPKO-sp3

Plasmid

#231850

Purpose

KO of adhP in Parageobacillus thermoglucosidasius

Depositor

Nico Claassens

Publication

Paredes-Barrada et al Metab Eng. 2025 Sep;91:145-157. doi: 10.

Insert

ThermoCas9, homology arms, and gRNA targetting adhP from Parageobacillus thermoglucosidasius

Use

CRISPR

Expression

Bacterial

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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pSpCas9-GFP UGT2 (pWZ587)

Plasmid

#254038

Purpose

Express SpCas9 and the UGT2 gRNA

Depositor

Jef Boeke

Publication

Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

gRNA_UGT2

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-PolB-gRNA-14-2-hspCas9

Plasmid

#187198

Purpose

gRNA-2 to target PolB exon 14.

Depositor

Robert Sobol

Publication

Fang et al Nucleic Acids Res. 2026 Apr 13;54(7):gka

Insert

PolB-gRNA insert

Use

CRISPR

Tags

N/A

Expression

Mammalian

Promoter

U6

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-PolB-gRNA-14-3-hspCas9

Plasmid

#187199

Purpose

gRNA-3 to target PolB exon 14.

Depositor

Robert Sobol

Publication

Fang et al Nucleic Acids Res. 2026 Apr 13;54(7):gka

Insert

PolB-gRNA insert

Use

CRISPR

Tags

N/A

Expression

Mammalian

Promoter

U6

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-PolB-gRNA-14-4-hspCas9

Plasmid

#187206

Purpose

gRNA-4 to target POLB exon 14.

Depositor

Robert Sobol

Publication

Fang et al Nucleic Acids Res. 2026 Apr 13;54(7):gka

Insert

PolB-gRNA insert

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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Topo_SpCas9.tracrRNA-CS1_AsCas12a DRv10

Plasmid

#237553

Purpose

TOPO vector for the cloning of the SpCas9 tracrRNA-CS1 - AsCas12a Direct Repeat (DR) v10 fragment into the pLCHKO_v4 hgRNA vector for scCHyMErA-Seq experiments.

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

SpCas9.tracrRNA-CS1_AsCas12a DRv10

Use

CRISPR

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-nCas9-PolI5M

Plasmid

#249066

Purpose

Expresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Publication

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

nCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-BFP (M39)

Plasmid

#239485

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Publication

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP

Use

CRISPR

Expression

Plant

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-cSRDX (M40)

Plasmid

#239486

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Publication

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-SRDX

Use

CRISPR

Expression

Plant

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 4)

Plasmid

#236609

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Publication

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-dCas9(sa)-VP64-AAV

Plasmid

#213035

Purpose

dCas9-VP64 expressed under control of TRE

Depositor

Nadav Ahituv

Publication

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

dCas9

Use

AAV and CRISPR

Tags

NLS and NLS-VP64

Promoter

TRE, miniCMV

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-2)

Plasmid

#236613

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Publication

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-E4-ABE-Cas9n-puro

Plasmid

#226586

Purpose

Express E4-ABE in mammalian cells

Depositor

Gang Wang

Publication

Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

Insert

E4-ABE

Use

CRISPR

Expression

Mammalian

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

Plasmid

#236612

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Publication

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

Plasmid

#236608

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Publication

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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