We narrowed to 10,862 results for: yeast
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Plasmid#174063PurposeExpresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.DepositorInsert2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
TagsFLAGExpressionYeastPromoterpCyc1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL15
Plasmid#174069PurposeExpresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
TagsFLAGExpressionYeastPromoterpGal1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)
Plasmid#173460PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen (pJJF003)
Plasmid#173459PurposeC-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)
Plasmid#173458PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
ExpressionYeastPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-U57A
Plasmid#169927PurposeWT U6 with a U57A mutationDepositorInsertU6 U57A
ExpressionBacterial and YeastAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cyterm-yotagRFPT pDRF1-GW
Plasmid#168090PurposeExpresses Cyterm-yotagRFPT to check for monomericity.DepositorInsertyotagRFPT
ExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cyterm-mGreenLantern pDRF1-GW
Plasmid#168091PurposeExpresses Cyterm-mGreenLantern to check for monomericity.DepositorInsertmGreenLantern
ExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMEL15_MATz
Plasmid#166099PurposeExpression of gRNA targeting the MAT locusDepositorInsertMAT Z1
UseCRISPRExpressionYeastAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP4
Plasmid#166106PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)DepositorAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP1
Plasmid#166103PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.DepositorAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP2
Plasmid#166104PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP9
Plasmid#166111PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2DepositorAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP3
Plasmid#166105PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP6
Plasmid#166108PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)DepositorAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP7
Plasmid#166109PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.DepositorAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS15_MATz
Plasmid#166100PurposeExpression of dual gRNA targeting the MAT locusDepositorInsertMAT Z1
UseCRISPRExpressionYeastAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG97
Plasmid#164849PurposePlasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t constructDepositorInsertPart of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
ExpressionYeastAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB ONLY R3
Plasmid#161511PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R3_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting R3 in the RIB1 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only