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pLX304 PCSK5 (T288P)-V5 blast

Plasmid

#83101

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLX304 PCSK5 (A270T)-V5 blast

Plasmid

#83102

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Alanine 270 to Threonine

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (H516P)-V5 blast

Plasmid

#83103

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Histidine 516 to Proline

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
BPK2013-BsCas12b

Plasmid

#121952

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b

Tags

10xHis tag and 3xHA tag

Expression

Bacterial

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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gamma2 UTR shRNA (shRNA4)

Plasmid

#120796

Purpose

shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

Depositor

Angel de Blas

Publication

Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

Insert

GABRG2 shRNA (Gabrg2 Rat)

Use

RNAi

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107321

Purpose

Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to SaCas9

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/68

Plasmid

#113921

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/68

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/66

Plasmid

#113920

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/66

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-LUCIDRS-MTX

Plasmid

#113925

Purpose

Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

LUCID_RS-MTX

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

Plasmid

#104619

Purpose

Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

Depositor

Tudor Fulga

Publication

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10

Tags

FLAG

Expression

Insect

Promoter

hsp70

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pG-HIV-SIN-2LTR

Plasmid

#104591

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Publication

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of self-inactivating HIV-based vectors

Use

Cloning vector

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 5kb promoter/AcGFP

Plasmid

#98101

Purpose

Zebrafish aBb crystallin 5kb promoter segment driving GFP expression

Depositor

Mason Posner

Publication

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 5 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 5 kb promoter fragment …

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 4kb promoter/AcGFP

Plasmid

#98100

Purpose

Zebrafish aBb crystallin 4kb promoter segment driving GFP expression

Depositor

Mason Posner

Publication

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 4 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 4 kb promoter fragment …

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SPA mimic WT

Plasmid

#92350

Purpose

To mimic SPA RNA processing and expression

Depositor

Ling-Ling Chen

Publication

Wu et al Mol Cell. 2016 Nov 3;64(3):534-548. doi:

Insert

SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLX302 PCSK5 (T288P)-V5 puro

Plasmid

#98709

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMT2 Cav2.2 DomI and DomII

Plasmid

#89891

Purpose

Mammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2

Depositor

Annette Dolphin

Publication

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

Cacna1b (CACNA1B )

Expression

Mammalian

Mutation

Stop codon after amino acid 1154 (this is a trunc…

Promoter

adenovirus major late promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1702

Plasmid

#78195

Purpose

optogenetic activation of IL2 neuron in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

ChR2(gf)

Expression

Worm

Promoter

lag-2

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pJL1603

Plasmid

#78193

Purpose

Ce cha-1 expression in SMB, SIA neurons in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

odr-2

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1602

Plasmid

#78192

Purpose

Ce cha-1 expression in SIA, RMD neurons in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

trp-1

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1601

Plasmid

#78191

Purpose

Ce cha-1 expression in IL2, SIA neurons in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

osm-6

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only