We narrowed to 15,991 results for: LEA

1. pTE4938

   Plasmid

   #107527

   Purpose

   Expresses Fn crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   mCherry
   

   Expression

   Mammalian

   Promoter

   CBh and human U6

   Available Since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4999

   Plasmid

   #107555

   Purpose

   Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(dead1)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   D917A

   Promoter

   CMV

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE4567

   Plasmid

   #107557

   Purpose

   Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(dead2)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   E1080A

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE4561

   Plasmid

   #107525

   Purpose

   Expresses Lb crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Lb crRNA
   mCherry
   

   Expression

   Mammalian

   Promoter

   CBh and human U6

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTE3320

   Plasmid

   #107529

   Purpose

   Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hLbCpf1(RVR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   G532R, K538V, Y542R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTE3314

   Plasmid

   #107530

   Purpose

   Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(RVR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K613V, N617R

   Promoter

   CMV

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE3319

   Plasmid

   #107533

   Purpose

   Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(RR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K637R

   Promoter

   CMV

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE3316

   Plasmid

   #107532

   Purpose

   Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(RR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K671R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE3317

   Plasmid

   #107531

   Purpose

   Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(RVR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K582V, N586R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE3179

    Plasmid

    #107528

    Purpose

    Expresses Mb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    mCherry
    

    Expression

    Mammalian

    Promoter

    CBh and human U6

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGreenII 0000 (No LUC)

    Plasmid

    #44465

    Depositor

    Roger Hellens

    Type

    Empty backbone

    Use

    Plant transformation

    Available Since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGreenII 0579-1 (35S::LUC)

    Plasmid

    #44468

    Depositor

    Roger Hellens

    Insert

    A luciferase gene
    

    Use

    Plant transformation

    Promoter

    35S Promoter

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAIP4_4QB4

    Plasmid

    #234187

    Purpose

    Heterologous protein expression of thermonuclease (TNase) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4QB4
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. C13m

    Plasmid

    #120960

    Purpose

    MoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank file

    Depositor

    Richard Murray

    Insert

    Cerulean
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZP06B

    Plasmid

    #232321

    Purpose

    Make sacB a counterselection marker to replace galK

    Depositor

    Chenggang Wu

    Insert

    PrpsJ-sacB
    

    Expression

    Bacterial

    Available Since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHEX2 T494 TALENs

    Plasmid

    #44464

    Depositor

    Roger Hellens, Ross Johnson

    Insert

    AtCRU3 T494 TALENs
    

    Use

    Plant transformation

    Promoter

    CaMV 35S

    Available Since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pBCG10

    Plasmid

    #232323

    Purpose

    Make mazF a counterselection marker to replace sacB in pZP06C

    Depositor

    Chenggang Wu

    Insert

    mazF-tetR
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. H2B CFP

    Plasmid

    #198059

    Purpose

    mCerulean fused to human H2B protein

    Depositor

    Amro Hamdoun

    Insert

    H2B
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mCerulean

    Promoter

    sp6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOpen-T5gene12/13

    Plasmid

    #165559

    Purpose

    Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    T5 Exonuclease
    

    Use

    Synthetic Biology

    Available Since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. C5V

    Plasmid

    #26394

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Insert

    Cerulean-5-Venus
    

    Use

    Fret standard

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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