Addgene

We narrowed to 31,038 results for: eng

Showing: 1021 - 1040 of 31038 results

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pACB110

Plasmid

#222209

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA (G258D)

Use

Tags

6x His

Expression

Bacterial

Mutation

Changed Gly258 to Aspartate

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Use

Tags

Expression

Bacterial

Mutation

A28C in the I-F repeat

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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(F)pGPD(G)_P2 (SBE110)

Plasmid

#194992

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pGPD (G)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pADH2 (J) P3 (SBE114)

Plasmid

#187629

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pADH2 (J)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(F) pADH2 (G)_P2 (SBE113)

Plasmid

#187628

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pADH2 (G)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

Plasmid

#195054

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLDP1

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

Plasmid

#195053

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pFBA

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

Plasmid

#195052

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

Plasmid

#195051

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pGPD

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

Plasmid

#195050

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Use

Tags

Expression

Bacterial

Mutation

Promoter

pADH2

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(F) pXYL (G) P1 (SBE216)

Plasmid

#195017

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pXYL (G)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(R) pXYL (D) P1 (SBE215)

Plasmid

#195016

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pXYL (D)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(R)pADH2(D)_P1(SBE214)

Plasmid

#195015

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(B) pADH2 (R)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(B) NAT marker (R) (SBE173)

Plasmid

#195014

Purpose

nourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+NAT+tNOS

Use

Tags

Expression

Bacterial

Mutation

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pGPD (J)_P3 (SBE111)

Plasmid

#194990

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pGPD (J)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(S) insD_ku70 (Q) (SBE139)

Plasmid

#187627

Purpose

the insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/Q

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(S) insD KU70 (Q)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(P) insUP_ku70 (SBE138) (B)

Plasmid

#187626

Purpose

the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(P) insUP KU70 (B)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLA2ScIfmTcIfm

Plasmid

#193792

Purpose

Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLLacO-1 and PLTetO-1

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRA1SEGFPTcIfm

Plasmid

#193777

Purpose

Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PR promoter and PLTetO-1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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