SS13 lac-inducible GFP reporter circuit
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30336||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerTom Knight, MIT Parts Registry
- Backbone size w/o insert (bp) 2079
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer Lac-Promoter (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byMIT Parts Registry
Terms and Licenses
- Not Available to Industry
Contains the wt lac promoter.
Please note that this plasmid, SS13, was not used in the associated publication (Sleight et al J Biol Eng. 2010). SS24 was used in the experiments described in this paper.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SS13 lac-inducible GFP reporter circuit was a gift from Herbert Sauro (Addgene plasmid # 30336 ; http://n2t.net/addgene:30336 ; RRID:Addgene_30336)
For your References section:Designing and engineering evolutionary robust genetic circuits. Sleight SC, Bartley BA, Lieviant JA, Sauro HM. J Biol Eng. 2010 Nov 1;4:12 10.1186/1754-1611-4-12 PubMed 21040586