We narrowed to 12,126 results for: LEA
-
Plasmid#216746PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL3/4sub
Plasmid#216747PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL3 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5/4sub
Plasmid#216748PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL1del
Plasmid#216749PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [FKISLAEM…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL2del
Plasmid#216750PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [WMGILKNN…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL3del
Plasmid#216751PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 3 (OL3) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
Tags10x HisExpressionBacterialMutationOuter loop 3 (OL3) with the sequence of [ELLGGPDS…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 mRuby K84ONBK
Plasmid#216226PurposeTDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.DepositorInsertTransactive response DNA binding protein of 43 kDa (TARDBP Human)
ExpressionMammalianMutationLysine 84 mutated to a TAG stop codonPromoterCMV PromoterAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
TagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt9 IRES mRuby3
Plasmid#195700PurposeLentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoterDepositorAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2A D197,199N
Plasmid#195704PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutationsDepositorInsertSyt9 (Syt5 Mouse)
TagsGSTExpressionBacterialMutationencodes amino acids 124-211 of mouse SYT9 with D1…Available SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HiFi SpCas9-NLS-6xHis
Plasmid#207378PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-Blackjack SpCas9-NLS-6xHis
Plasmid#207375PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertBlackjack SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationamino acids 1005-1013 replaced with two glycinePromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mhc2dab:mCherry
Plasmid#68997Purposefor making transgenic fishDepositorAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-20b_HtrA2(delta(133))_S306A_6His
Plasmid#197975PurposeE. coli expression of HtrA2(delta133)-6HisDepositorAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPTI-21
Plasmid#195058Purposesimplyfied amino acid sequence by alanine scanningDepositorInsertbovine pancreatic trypsin inhibitor
TagsHis tag and trpΔLE leader sequencesExpressionBacterialMutation21 amino acids are Alanine out of 58 amino acidsPromoterT7 promotorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-G132EL133EP134RT137R-dsRNAres_Y
Plasmid#148109PurposeInsect Expression of DmPAN2-G132EL133E134RT137R-dsRNAresDepositorInsertDmPAN2-G132EL133E134RT137R-dsRNAres (PAN2 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F99E-dsRNAres_Y
Plasmid#148072PurposeInsect Expression of DmPAN2-F99E-dsRNAresDepositorInsertDmPAN2-F99E-dsRNAres (PAN2 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F99EG132EL133E-dsRNAres_Y
Plasmid#148073PurposeInsect Expression of DmPAN2-F99EG132EL133E-dsRNAresDepositorInsertDmPAN2-F99EG132EL133E-dsRNAres (PAN2 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F550EM554EK557E-dsRNAres_Y
Plasmid#148074PurposeInsect Expression of DmPAN2-F550EM554EK557E-dsRNAresDepositorInsertDmPAN2-F550EM554EK557E-dsRNAres (PAN2 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-G132EL133E-dsRNAres_Y
Plasmid#148076PurposeInsect Expression of DmPAN2-G132EL133E-dsRNAresDepositorInsertDmPAN2-G132EL133E-dsRNAres (PAN2 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only