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pMiR-CIP2A(partial)

Plasmid

#53705

Purpose

Luciferase reporter assay for microRNA binding on CIP2A coding sequence

Depositor

Edward Chan

Publication

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Available since

Aug. 28, 2014

Availability

Academic Institutions and Nonprofits only
pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52542

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

Plasmid

#52878

Purpose

Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

Depositor

Thomas Oertner

Publication

Wietek et al Science. 2014 Mar 27.

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E90R, D156N, T159C

Promoter

CaMKIIa

Available since

July 16, 2014

Availability

Academic Institutions and Nonprofits only

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pRVdG-mCherry-N-P-M-nucBFP-EGFP-L

Plasmid

#52484

Purpose

Expresses mCherry, nuclear mTagBFP and EGFP

Depositor

Sebastian Seung

Publication

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mCherry
nuclear mTagBFP
EGFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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psbA2-PHLS (b)

Plasmid

#52307

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Publication

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

psbA2

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pWPT-/UAA-mEGFP-IRES-mCherry

Plasmid

#49234

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Publication

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

EF1-short

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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AU1-TTC30B-pcDNA3

Plasmid

#47325

Purpose

Expresses AU1 epitope-tagged TTC30B in mammalian cells

Depositor

Richard Maurer

Publication

Howard et al Exp Cell Res. 2013 Jun 25. pii: S0014-48

Insert

TTC30B (Ttc30b Mouse)

Tags

AU1 epitope

Expression

Mammalian

Promoter

CMV

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS

Plasmid

#43969

Depositor

Jerry Crabtree

Publication

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Gal4/DBD

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Bcr DH domain pGEX

Plasmid

#38179

Depositor

Nora Heisterkamp

Publication

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain (BCR Human)

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S

Plasmid

#40394

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2161,C2024S/C2025S

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBABE hygro MEN1 P12L

Plasmid

#11020

Depositor

Matthew Meyerson

Publication

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 P12L (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

P12L

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 A309P

Plasmid

#11023

Depositor

Matthew Meyerson

Publication

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 A309P (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A309P

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D2 K112E HA

Plasmid

#8953

Depositor

Philip Hinds

Publication

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D2 (CCND2 Human)

Tags

HA

Expression

Mammalian

Mutation

K112E: point mutation in cyclin box

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
MAPGW

Plasmid

#205696

Purpose

shRNAmir backbone for RNA interference, with GFP reporter

Depositor

Dapeng Wang

Publication

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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MAPTW

Plasmid

#205695

Purpose

shRNAmir backbone for RNA interference, with mTagBFP2 reporter

Depositor

Dapeng Wang

Publication

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB2792

Plasmid

#63653

Purpose

pCfB2792 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LMAPCW

Plasmid

#205655

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Publication

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LCAPCW

Plasmid

#205646

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Publication

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

hCMV

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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LMAPPW

Plasmid

#205657

Purpose

shRNAmir backbone for RNA interference, with Puromycin selection

Depositor

Dapeng Wang

Publication

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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