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pST_SAHS4_k1_0 (pBS0769)

Plasmid

#185249

Purpose

For the mammalian expression of the tardigrade protein SAHS4_k1_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

SAHS4_k1_0

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1386-1535_S

Plasmid

#147540

Purpose

Mammalian Expression of HsNot1iso1_1386-1535

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_1386-1535 (CNOT1 Human)

Expression

Mammalian

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R

Plasmid

#147433

Purpose

Insect Expression of DmNot1_1711-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1711-2505-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1148_I

Plasmid

#146604

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Expression

Insect

Mutation

4 silent and two non silent N605S, K759M mutation…

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-optimized E6AP F727D

Plasmid

#165101

Purpose

ecoli optimized expression of a GST fusion of Human E6AP F727D

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

Insert

E6AP F727D (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMtb MCM

Plasmid

#138296

Purpose

Expression of Mtb MCM small and large subunit

Depositor

Ruma Banerjee

Article

Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

Inserts

mutA
mutB

Tags

His tag and TEV cleavage site

Expression

Bacterial

Mutation

inserted TEV cleavage site between His-tag and m…

Promoter

T7

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4F07

Plasmid

#71056

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Mi-2 (Mi-2 Fly)

Use

Gateway Cloning

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G05

Plasmid

#70809

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIbeta (CkIIbeta Fly)

Use

Gateway Cloning

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B07

Plasmid

#70819

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIalpha (CkIIalpha Fly)

Use

Gateway Cloning

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO myc Separase delta 1278-1291

Plasmid

#59830

Purpose

Allows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 1278-1291

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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PT7-alphaHL

Plasmid

#227650

Purpose

pT7-driven expression of alpha-hemolysin, a pore-forming protein in lipid bilayers.

Depositor

Richard Murray

Insert

alpha-hemolysin

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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CAHS2parrc-mCh-2xFKBP (pBS1109)

Plasmid

#185304

Purpose

For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2parrc

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-MEK1_fusion

Plasmid

#39194

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pRetroX-TRE3G_NT-GSDMD

Plasmid

#201170

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-L4A-MEK1_fusion

Plasmid

#39197

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)

Tags

Myc

Expression

Mammalian

Mutation

L4A = L33A, L37A, L40A, L42A

Promoter

CMV

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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p11d-aRPA (MSW#712)

Plasmid

#208078

Purpose

Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLgw V5-EcoDam

Plasmid

#59210

Purpose

Mammalian DamID control lentiviral vector for expression of V5-Dam only

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Insert

EcoDam

Use

Lentiviral; Damid

Tags

V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter and heat shock minima…

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-MyosinIIC-N-18

Plasmid

#55108

Purpose

Localization: Cytoskeleton, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only