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pET30-T7-His-sfGFP

Plasmid

#180755

Purpose

pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Publication

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJH579

Plasmid

#172163

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD JM10.1); AraC

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH462

Plasmid

#172154

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Sumo-tagged pylBCD); AraC

Expression

Bacterial

Mutation

SUMO tag fused to N-terminus of PylB

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH577

Plasmid

#172161

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2); AraC

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylD…

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH19

Plasmid

#172141

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*)

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHR Fyn-TagBFP

Plasmid

#171035

Purpose

TagBFP-tagged Fyn kinase

Depositor

Jared Toettcher

Publication

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Fyn

Use

Lentiviral

Expression

Mammalian

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1C-shSCR

Plasmid

#139472

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Publication

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pM116: CasRx VEGFA presgRNA

Plasmid

#166868

Purpose

U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Publication

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

Human VEGFA targeting presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM62624

Plasmid

#169094

Purpose

Low copy MoClo level 2 binary vector for plant transformation

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Expression

Plant

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM49981

Plasmid

#169087

Purpose

MoClo level 0 BvADHα coding sequence module

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvADHα

Use

Synthetic Biology; Moclo cloning vector

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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dGFP-Rabphilin

Plasmid

#170866

Purpose

Mammalian expression of dGFP-Rabphilin.

Depositor

Justin Taraska

Publication

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rabphilin-3a (RPH3A Bovine)

Tags

dEGFP

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM921

Plasmid

#169086

Purpose

MoClo level 0 Db5GT coding sequence module

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Db5GT

Use

Synthetic Biology; Moclo cloning vector

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pFMD

Plasmid

#160921

Purpose

Formate dehydrogenase (FMD) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Formate dehydrogenase promoter

Use

Synthetic Biology

Mutation

BsaI and BbsI domesticated

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pCAT1

Plasmid

#160918

Purpose

Catalase 1 (CAT1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Catalase 1 promoter

Use

Synthetic Biology

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAG499-FRB-N-frFAST

Plasmid

#160059

Purpose

Expresses FRB-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FRB-N-frFAST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAG505-H2B-frFAST

Plasmid

#160056

Purpose

Expresses H2B-frFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

H2B-frFAST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050G (white)

Plasmid

#132420

Purpose

express arrays of gRNA targeting White under dU6-3 promoter

Depositor

Omar Akbari

Publication

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

white gRNA array

Expression

Insect

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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OA-1050J (GFP)

Plasmid

#133304

Purpose

express arrays of gRNA targeting GFP under dU6-3 promoter

Depositor

Omar Akbari

Publication

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

GFP gRNA array

Expression

Insect

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJAZ10::LUC

Plasmid

#141172

Purpose

Luminescent reporter for jasmonic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Publication

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtJAZ10 promoter

Use

Luciferase

Expression

Plant

Promoter

AtJAZ10, AT5G13220

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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