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pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

Plasmid

#173454

Purpose

Contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPAiRFP1-N1

Plasmid

#44272

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

Insert

PAiRFP1

Expression

Mammalian

Mutation

G127D/S141R/M163L/Q168L/A203V/G218S/R220P/V244F/A…

Promoter

CMV

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby-Kan

Plasmid

#44956

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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PKC beta II CAT

Plasmid

#16384

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, aa 329-673

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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mEos2-H2B-6

Plasmid

#57384

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H2B (H2BC11 Human)

Tags

mEos2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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APPL1-pmCherryC1

Plasmid

#27683

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

APPL1 (Appl1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
Rc/CMV cyclin D3

Plasmid

#10912

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D3 (CCND3 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pTRANS_212

Plasmid

#91111

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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LT3GEN

Plasmid

#111173

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Promoter

T3G

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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AP-SNAP25

Plasmid

#167995

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

SNAP25-T7PAP

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoSuperfolderGFP-SpHis5

Plasmid

#44837

Purpose

Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoSuperfolderGFP

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pECFP-DEVG-Venus

Plasmid

#24538

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVG-Venus

Tags

ECFP and Venus

Expression

Mammalian

Mutation

DEVDR mutated to DEVG

Promoter

CMV

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
Skylan-S-(GGGGS)x3-β-Actin

Plasmid

#128938

Purpose

fluorescnece reporter of β-Actin with Skylan-S. Express in mammalian cells

Depositor

Shimon Weiss

Article

Yi et al Biomed Opt Express. 2019 Apr 16;10(5):24

Insert

Skylan-S

Tags

β-actin

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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H2B-LSSmGFP

Plasmid

#186539

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Human Histone 2B (D26G and V119I mutations)

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pmKalama1-Nuc

Plasmid

#14894

Purpose

Mammalian expression of mKalama1 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pmKalama1-Nuc

Tags

NLS

Expression

Mammalian

Mutation

I178T

Promoter

CMV

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pRSII42H-TDH3pr-mNeon

Plasmid

#194539

Purpose

High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAG460-FRB-greenNFAST

Plasmid

#160361

Purpose

Expresses FRB-greenNFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

FRB-greenNFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pKeratin-LSSmOrange

Plasmid

#37138

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytokeratin 18

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

Plasmid

#173455

Purpose

Contains mScarlet-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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hTRIM65_EGFP_N1

Plasmid

#54320

Purpose

Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

TRIM65 (TRIM65 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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