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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,321 results for: LEA

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Showing: 10841 - 10860 of 12321 results

  1. TBXTA-c024

    Plasmid
    #139761
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-D225
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139761-plasmid-map-sequence-id-296544

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  2. pGBW-m4046234

    Plasmid
    #149053
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF7b
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF7b (ORF7b Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    May 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149053-plasmid-map-sequence-id-296273

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  3. pGBW-m4046273

    Plasmid
    #145480
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145480-plasmid-map-sequence-id-287657

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  4. pGBW-m4046550

    Plasmid
    #145463
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp9
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145463-plasmid-map-sequence-id-287640

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  5. pGBW-m4046519

    Plasmid
    #145397
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp11
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145397-plasmid-map-sequence-id-287574

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  6. pGBW-m4046257

    Plasmid
    #145393
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145393-plasmid-map-sequence-id-287570

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  7. pGBW-m4046185

    Plasmid
    #145508
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145508-plasmid-map-sequence-id-292509

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  8. pTone-gata2aECE-nsGFP

    Plasmid
    #132975
    Purpose
    gata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone
    Depositor
    Nathan Lawson
    Publication
    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d
    Inserts
    nuclear localized sfGFP
    gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
    Use
    Unspecified; Transposon-mediated
    Tags
    6x myc and SV40 NLS
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    132975-plasmid-map-sequence-id-264810

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  11. pSBbi-Pur-Lyn-AktAR2

    Plasmid
    #125197
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Publication
    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:
    Insert
    Lyn-AktAR2
    Use
    Transposon
    Tags
    Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125197-plasmid-map-sequence-id-243582

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  12. 6DUB (LOC149281)

    Plasmid
    #119967
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    LOC149281 (METTL11B Human)
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acids 58-278
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119967-plasmid-map-sequence-id-232691

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  13. WNK3 (WNK3B-c005)

    Plasmid
    #110262
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    WNK3 (WNK3 Human)
    Tags
    His-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110262-plasmid-map-sequence-id-212340

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  14. WNK3 (WNK3B-c001)

    Plasmid
    #110261
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    WNK3 (WNK3 Human)
    Tags
    His-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110261-plasmid-map-sequence-id-212339

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  15. FOXN1 (FOXN1A-c017)

    Plasmid
    #110249
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FOXN1 (FOXN1 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110249-plasmid-map-sequence-id-212285

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  16. WNK2 (WNK2A-c003)

    Plasmid
    #110250
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    WNK2 (WNK2 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110250-plasmid-map-sequence-id-212284

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  18. pDD2024

    Plasmid
    #101368
    Purpose
    Name: pCBG68-RBPjkΔA. RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).
    Depositor
    Dirk Dittmer
    Publication
    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1
    Insert
    RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).
    Use
    Luciferase
    Mutation
    RBPjk binding site A mutation in pCBG68-LANApi (p…
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101368-plasmid-map-sequence-id-195304

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Showing: 10841 - 10860 of 12321 results