We narrowed to 15,822 results for: LEA

1. H2B-mCherry

   Plasmid

   #108880

   Purpose

   Expression of human histone2B fused to mCherry (nuclear marker)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   H2B-mCherry
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST14-DoubleCatcher-H-Lock

   Plasmid

   #216285

   Purpose

   Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher H-Lock
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-CaNAR2

   Plasmid

   #64728

   Purpose

   FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNAR2
   

   Use

   Tags

   Cerulean3 and YPet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-StagRFP

   Plasmid

   #173014

   Purpose

   Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   StagRFP
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-OSF-PP

   Plasmid

   #154247

   Purpose

   backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Type

   Empty backbone

   Use

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5UAS zfSynaptophysin:GFP

   Plasmid

   #74316

   Purpose

   A fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeats

   Depositor

   Stephen Smith, Martin Meyer

   Article

   Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

   Insert

   synaptophysin
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Q12H and deletion of F13 (please see depositor co…

   Promoter

   5UAS

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AviTag-SpyCatcher

   Plasmid

   #72326

   Purpose

   Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   AviTag-SpyCatcher
   

   Use

   Tags

   N-term His6 tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a - NR

   Plasmid

   #89625

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Nitrate reductase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZY101

   Plasmid

   #73932

   Purpose

   This binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.html

   Depositor

   Zhanyuan Zhang

   Article

   Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

   Insert

   bar
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. UAS-Syt::Halo7

    Plasmid

    #87648

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::Halo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::Halo7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. KLHL40 (KBTBD5A-c006)

    Plasmid

    #98233

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KBTBD5A (KLHL40 Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBA2675

    Plasmid

    #189997

    Purpose

    Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)

    Depositor

    Bungo Akiyoshi

    Article

    Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

    Inserts

    FBP75
    VhhGFP4
    

    Use

    Expression vector for trypanosoma brucei

    Tags

    NLS

    Expression

    Mutation

    N-terminal 600 bp of FBP75

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 14UAS zfPSD95:GFP

    Plasmid

    #74314

    Purpose

    A fusion of zebrafish PSD95 to GFP under control of 14 UAS repeats

    Depositor

    Stephen Smith, Martin Meyer

    Article

    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

    Insert

    zebrafish PSD95
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Y599H and I793S (please see depositor comment bel…

    Promoter

    UAS

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCRISPR-S12

    Plasmid

    #84031

    Purpose

    To episomally express codon optimized Cas9 and chimeric guide RNA

    Depositor

    Sen Wu

    Article

    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

    Inserts

    hSpCas9
    eGFP
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (downstream of F2A self-cleaving pept…

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1-jGCaMP7b-XN

    Plasmid

    #178362

    Purpose

    Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.

    Depositor

    Xiaodong Liu

    Article

    Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

    Insert

    jGCaMP7b-XN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-H2B_Halo_T2A_EGFP

    Plasmid

    #135444

    Purpose

    Mamalian cell expression of nuclear HaloTag7 and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    H2B-HaloTag7 and EGFP
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. VKG1-gRNA-pX330

    Plasmid

    #108671

    Purpose

    Cleavage of VKG1 sequence by CRISPR/Cas9

    Depositor

    Shigeo Sugano

    Article

    Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

    Insert

    gRNA for VKG1 sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. UtB2N7

    Plasmid

    #161015

    Purpose

    ubiquitously expressed nuclear localized tagBFP2

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    tagBFP2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRGD-TcR

    Plasmid

    #74110

    Purpose

    Plasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Insert

    tetA(C)
    

    Use

    Bacterial vector based on pmb1 replicon

    Tags

    Expression

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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