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CKMT2 gRNA (BRDN0001147460)

Plasmid

#76596

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKMT2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKMT2 (CKMT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKMT2 gRNA (BRDN0001148542)

Plasmid

#76597

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKMT2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKMT2 (CKMT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IRAK1 gRNA (BRDN0001145800)

Plasmid

#76418

Purpose

3rd generation lentiviral gRNA plasmid targeting human IRAK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IRAK1 (IRAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TGFBR3 gRNA (BRDN0001144841)

Plasmid

#76292

Purpose

3rd generation lentiviral gRNA plasmid targeting human TGFBR3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TGFBR3 (TGFBR3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NEK6 gRNA (BRDN0001147000)

Plasmid

#76256

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK6

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK6 (NEK6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PASK gRNA (BRDN0001145672)

Plasmid

#76203

Purpose

3rd generation lentiviral gRNA plasmid targeting human PASK

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PASK (PASK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LIMK1 gRNA (BRDN0001147777)

Plasmid

#76049

Purpose

3rd generation lentiviral gRNA plasmid targeting human LIMK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LIMK1 (LIMK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ATMIN gRNA (BRDN0001148346)

Plasmid

#75861

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATMIN

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ATMIN (ATMIN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK15 gRNA (BRDN0001162376)

Plasmid

#75620

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NUAK1 gRNA (BRDN0001145557)

Plasmid

#75575

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK1 (NUAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BCKDK gRNA (BRDN0001149253)

Plasmid

#75505

Purpose

3rd generation lentiviral gRNA plasmid targeting human BCKDK

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BCKDK (BCKDK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GSG2 gRNA (BRDN0001145625)

Plasmid

#77520

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSG2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSG2 (HASPIN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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INSRR gRNA (BRDN0001487067)

Plasmid

#78062

Purpose

3rd generation lentiviral gRNA plasmid targeting human INSRR

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

INSRR (INSRR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestGFP_RBPMS

Plasmid

#65744

Purpose

expresses GFP-tagged RBPMS in mammalian cells

Depositor

Thomas Tuschl

Publication

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestRFP_RBPMS

Plasmid

#65745

Purpose

expresses RFP-tagged RBPMS in mammalian cells

Depositor

Thomas Tuschl

Publication

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

RFP

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pScalps_puro_mIL-2Rα RK

Plasmid

#59920

Purpose

Expresses mouse interleukin-2 receptor alpha chain mutated

Depositor

Silvia Monticelli

Publication

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Mutation

changed sequence of the intracellular (C-terminal…

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Btg1

Plasmid

#45865

Depositor

Jeffrey Macklis

Publication

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Btg1 in situ probe (Btg1 Mouse)

Use

In situ

Mutation

contains bp# 1004-1638 of BC006834.1

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
YFP Gamma 10

Plasmid

#36108

Depositor

Narasimhan Gautam

Publication

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 10 (Gng10 Mouse)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.1puro-shWillin-B

Plasmid

#40886

Depositor

Paul Reynolds

Publication

Angus et al Oncogene. 2012 Jan 12;31(2):238-50. doi:

Insert

shWillin-B (FRMD6 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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p172 Dync1i2.E (Ex 1b)

Plasmid

#26442

Depositor

Elizabeth Fisher

Publication

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform E (exon 1b) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available since

March 24, 2011

Availability

Academic Institutions and Nonprofits only