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pG20_TEV_MYC_FR

Plasmid

#240666

Purpose

Expression plasmid for TEV-MYC-tagged proteins with FAST-Red transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-MYC

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_HA_FR

Plasmid

#240667

Purpose

Expression plasmid for TEV-HA-tagged proteins with FAST-Red transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-HA

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_Flag_FR

Plasmid

#240668

Purpose

Expression plasmid for TEV-Flag-tagged proteins with FAST-Red transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-Flag

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CrPV-IRES-cytERM-SUNTAG-MS2

Plasmid

#247299

Purpose

Mammalian expression of ER-specific SUNTAG reporter: piggyBac vector expressing cytERM-SUNTAG MS2; 5’ UTR includes cricket paralysis virus IRES, allowing escape from translation initiation regulation.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

PB-CrPV-IRES-cytERM-SUNTAG-MS2

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-N pc4789

Plasmid

#229753

Purpose

Bacterial expression plasmid of MBD2-N (N-terminus of MBD2: aa 1-152). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

N-terminus of MBD2: aa 1-152

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-MBDTRD pc4791

Plasmid

#229754

Purpose

Bacterial expression vector of MBD2-MBDTRD domains (aa 153-235) C-terminal tagged to the intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2-MBDTRD domains (aa 153-235)

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆C pc4792

Plasmid

#229755

Purpose

Bacterial expression plasmid of MBD2∆C lacking the C-terminus (a 236-414). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the C-terminus (a 236-414)

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-C pc4793

Plasmid

#229756

Purpose

Bacterial expression plasmid of MBD2-C (C-terminus of MBD2: aa 236-414). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

C-terminus of MBD2: aa 236-414

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2∆C-G pc4794

Plasmid

#229757

Purpose

Mammalian expression vector of MBD2. The Protein is lacking the C-terminus: aa 236-414. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

MBD2 lacking the C-terminus: aa 236-414.

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆N(MBD2b) pc4787

Plasmid

#229752

Purpose

Bacterial expression plasmid of MBD2∆N(MBD2b) lacking the N-terminus: aa 1-152. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the N-terminus: aa 1-152.

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1543

Plasmid

#246062

Purpose

Bacterial expression of mutant T4 Acb2 (Y8A) under control of the pJex promoter

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Anti CBASS 2

Expression

Bacterial

Mutation

Y8A

Promoter

pJEX promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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ED_p_060

Plasmid

#246070

Purpose

ECOR31 mCpol bacterial expression under T7 promoter, C-term TEV-MBP-HIS10

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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ED_p_044a

Plasmid

#246071

Purpose

ECOR31 mCpol bacterial expression under T7 promoter, N-term His10-MBP-TEV

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-RILPL1 (R293A)

Plasmid

#244981

Purpose

Express RILPL1 in mammalian cells with mCherry tag expressing a R293A mutation

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

RILPL1 (RILPL1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R293A

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDCX_mEmerald_CRISPR_HDR

Plasmid

#239331

Purpose

HDR template to insert mEmerald before the stop codon in exon 7 of the human doublecortin (DCX) locus

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

mEmerald and DCX homology arms

Use

CRISPR

Promoter

None

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmEmerald_mEmerald_I3

Plasmid

#231681

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with two tandem mEmeralds in mammalian cells. Assembles into nanocages tagged with 120 mEmerald FPs.

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

I3-01

Tags

mEmerald

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmEmerald_I3

Plasmid

#231680

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with one mEmerald in mammalian cells. Assembles into nanocages tagged with 60 mEmerald FPs.

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

I3-01

Tags

mEmerald

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-hPLEKHA6-Myc

Plasmid

#236540

Purpose

For expression of PLEKHA6 (human) in mammalian cells

Depositor

Sandra Citi

Article

Sluysmans et al Mol Biol Cell. 2021 Nov 1;32(21):ar34. d

Insert

PLEKHA6 (PLEKHA6 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-FL

Plasmid

#216515

Purpose

Expresses MBP-tagged full length hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2 FL

Tags

6xHis tags-MBP

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pETG20a-Salro-HMG

Plasmid

#231543

Purpose

Expresses the HMG of Salro Sox-like, for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-Iike HMG

Tags

6xHis

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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