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aav-tnt-actn2-gfp

Plasmid

#165034

Purpose

AAV-based delivery of ACTN2-GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

ACTN2 (Actn2 Mouse)

Use

AAV

Tags

GFP

Promoter

cTNT

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Budding Yeast, Synthetic, Human)

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGEN-WPRE-bGH-DEST (JDW 1309)

Plasmid

#229815

Purpose

A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2A

Plasmid

#202732

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (PIP4K2A, FKBP1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-INPP4BC842A

Plasmid

#116865

Purpose

Inactive recruitable PI(3,4)P2 4-phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

INPP4B (INPP4B Human)

Tags

FKBP12 and mCherry

Expression

Mammalian

Mutation

catalytic cysteine 842 changed to alanine

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

Plasmid

#98930

Purpose

AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11C(E184Q)-HA

Plasmid

#209251

Purpose

Mammalian expression of ATP11C mutant E184Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Tags

HA

Expression

Mammalian

Mutation

E184Q

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8A1(E191Q)-HA

Plasmid

#209240

Purpose

Mammalian expression of ATP8A1 mutant E191Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Tags

HA

Expression

Mammalian

Mutation

E191Q

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3-MAP4-MTBD

Plasmid

#171498

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

So et al Science. 2019 Jun 28;364(6447). pii: 364

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mClover3

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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ROSA26(MultiFPsΔPuro)

Plasmid

#140759

Purpose

Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Inserts

puromycin-N-acetyltransferase
Venus
mCherry
mCerulean

Use

Mouse Targeting

Promoter

CAGGS

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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Arf6 T27N- Venus in pcDNA3.1

Plasmid

#90464

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

Castillo-Badillo et al ACS Synth Biol. 2020 Mar 16. doi: 10.102

Insert

Arf6 T27N (ARF6 )

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7a-7

Plasmid

#56443

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab7a (RAB7A Human)

Tags

EGFP

Expression

Mammalian

Mutation

N117S in NM_004637.5

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1-CHRFAM7A-YFP

Plasmid

#62634

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

YFP inserted in M3-M4 loop

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pX330-RAB7A

Plasmid

#227297

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of RAB7A (RAB7A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-moxGFP-Zeo-H2BC11

Plasmid

#207759

Purpose

Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 17, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mEosEM-E_Lamin A

Plasmid

#226338

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Insert

Lamin A (LMNA Human)

Tags

mEosEM-E

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-GBF1(E794K)

Plasmid

#206416

Purpose

Mammalian expression of hGBF1(E794K)

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Tags

HA

Expression

Mammalian

Mutation

E794K

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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ACE2(-202)-luc

Plasmid

#31112

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -202 to +103

Available Since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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Mini-Ii-Str_SBP-EGFP-LIMP2

Plasmid

#222324

Purpose

Synchronize the trafficking of LIMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet3-Giantin (JDW 1356)

Plasmid

#224502

Purpose

Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-Giantin

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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