We narrowed to 14,841 results for: flag
-
Plasmid#223382PurposeT-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpRY-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT03
Plasmid#223375PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT07
Plasmid#223379PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry
Plasmid#230043PurposePartial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.DepositorInsertlox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
UseCre/Lox; Zebrafish expressionPromoterfabp10aAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-SpdCas9-Dnmt3A/3L-WT
Plasmid#222502PurposeExpresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherryDepositorInsertsS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
mCherry
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterTRE3GV and hPGKAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 TIFA T9A
Plasmid#231590PurposeTIFA gene mutated in position T9A (mutation on phosphorylation site) under control of a doxycycline-inducible promoterDepositorInsertTIFA (TIFA Human)
UseLentiviralTags3X flagExpressionMammalianMutationT9APromoterdoxycyclineAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 TIFA WT
Plasmid#231589PurposeTIFA WT gene under control of a doxycycline-inducible promoterDepositorAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 ALPK1 V1092A
Plasmid#231588PurposeALPK1 gene mutated in position V1092A (mutation found in spiradenomacarcinoma (sweat gland cancer)) under control of a doxycycline-inducible promoterDepositorInsertALPK1 (ALPK1 Human)
UseLentiviralTags3X flagExpressionMammalianMutationchanged Valine1092 to AlaninePromoterdoxycyclineAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZZMP26
Plasmid#231755PurposeMammalian expression of TOP6BLDepositorAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
4G-Cloning Plasmid Kit
Plasmid Kit#1000000252PurposeUse 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial ExpressionCloning TypeGolden GateAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJCX3-ACTG2_R257C
Plasmid#228911PurposeExpresses mutant R257C smooth muscle actin gamma-2 in human cellsDepositorInsertActin gamma 2, smooth muscle (ACTG2 Human)
TagsHIS-FLAG-TEVExpressionBacterial and MammalianMutationR257CPromoterCMVAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJCX3-ACTG2_WT
Plasmid#228910PurposeExpresses wildtype smooth muscle actin gamma-2 in human cellsDepositorInsertActin gamma 2, smooth muscle (ACTG2 Human)
TagsHIS-FLAG-TEVExpressionBacterial and MammalianMutationWTPromoterCMVAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMH-HA/Joseph2-Multitagged Plasmid
Plasmid#226726PurposeValidates the expression and detection of epitope-tagged proteins of interest in mammalian cells.DepositorInsertpMH-HA/Joseph2
TagsHA, Myc, GFP, Flag, V5, HisExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCV-FKBP-SYNDAPIN2
Plasmid#221311PurposeExpression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transductionDepositorAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-SopE
Plasmid#221327PurposeDox-inducible expression of SopE in mammalian cells by retroviral transductionDepositorInsertSopE
UseRetroviralTagsFLAGPromoterTRE3GVAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-MAVS(pLxIS)
Plasmid#221256PurposeExpression of MAVS pLxIS (393-441) in mammalian cells by retroviral transductionDepositorAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-APOBEC1
Plasmid#209039PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLSDepositorInsertAPOBEC1-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLM433
Plasmid#217293PurposeFor creating CyanoTag vectors by Golden Gate cloning (BspQI version)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmNeonGreen - 3x FLAGAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-FFSS-Cyclin D1
Plasmid#215146PurposeExpresses FFSS-Cyclin D1 in mammalian cells from an inducible lentiviral vector.DepositorInsertCCND1 (CCND1 Human)
UseLentiviralTagsFFSS (FLAG-FLAG-STREP-STREP)ExpressionMammalianPromoterTRE3GSAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-FFSS-Cyclin D1 (T286A)
Plasmid#215152PurposeExpresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.DepositorInsertCCND1 (CCND1 Human)
UseLentiviralTagsFFSS (FLAG-FLAG-STREP-STREP)ExpressionMammalianMutationWith T286A mutation.PromoterCMVAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only