We narrowed to 15,981 results for: LEA

1. pCS2-Cer.zf1

   Plasmid

   #61389

   Purpose

   mRNA synthesis of cerulean codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cer.zf1
   

   Use

   Zebrafish expression

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Spy0469

   Plasmid

   #159996

   Purpose

   Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   Spy0469 (24-389)
   

   Tags

   His6-tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Talin Head

   Plasmid

   #186140

   Purpose

   For expression of recombinant Talin head domain in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   TLN1 (TLN1 Human)
   

   Tags

   His6 (tev cleavage)

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS1-rab-3P::his-24::tagBFP

   Plasmid

   #124343

   Purpose

   Nuclear Localized Pan-Neuronal tagBFP

   Depositor

   Andrew Leifer

   Article

   Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

   Insert

   rab-3, tagBFP (rab-3 Nematode)
   

   Expression

   Worm

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA-FRT-FAK0

   Plasmid

   #45047

   Purpose

   FAK fret sensor without helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. PH-dL5**

   Plasmid

   #96946

   Purpose

   PLC pleckstrin homology domain linked to FAP for CALI validation

   Depositor

   Marcel Bruchez

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   Pleckstrin homology domain
   

   Tags

   dL5 and mCerulean

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. NLS-FUS-Citrine-PixE

   Plasmid

   #111506

   Purpose

   For the nuclear localization of the PixELL system

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

   Insert

   PixE (slr1693 )
   

   Use

   Lentiviral

   Tags

   Citrine, FUS, and NLS

   Promoter

   SFFV

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEos3.2-LaminA-C-18

   Plasmid

   #57463

   Purpose

   Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   LaminA (LMNA Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pNUT3

   Plasmid

   #175744

   Purpose

   Single-component photo-inducible (CRY2-based) nucleolus-targeting tool 3

   Depositor

   Yubin Zhou

   Article

   Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

   Insert

   mCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CHD1 (CHD1A-c107)

    Plasmid

    #110253

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CHD1 (CHD1 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL50015

    Plasmid

    #50318

    Purpose

    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mNEON (Branchistoma lanceolatum)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rap-MESA-20-M-Gal4

    Plasmid

    #84554

    Purpose

    MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

    Depositor

    Joshua Leonard

    Article

    Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

    Insert

    Rap-MESA-FRB-20-M-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME nls-eos

    Plasmid

    #73791

    Purpose

    Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

    Depositor

    David Raible

    Article

    Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

    Insert

    nls-eos
    

    Use

    Middle entry clone

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15-MTFmt

    Plasmid

    #31174

    Depositor

    Linda Spremulli

    Article

    Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

    Insert

    Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
    

    Tags

    His and thrombin cleavage

    Expression

    Bacterial

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pRDA_275

    Plasmid

    #179091

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-NG
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-pOTC-3H-GFP

    Plasmid

    #205725

    Purpose

    Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    pOTC-3H-GFP
    

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4046538

    Plasmid

    #149128

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBlue-TAL

    Plasmid

    #49401

    Purpose

    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

    Depositor

    Michal Zurovec

    Article

    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

    Type

    Empty backbone

    Use

    TALEN

    Expression

    Insect

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVITRO1-iRFP702-LaminB1

    Plasmid

    #184456

    Purpose

    Nuclear membrane-targeted iRFP702 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    iRFP702-LaminB1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCY 3010-02

    Plasmid

    #36210

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    yeast-enhanced (yE) polylinker
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-Cerulean-hphMX4

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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