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pBAD_His B_QuasAr1

Plasmid

#64135

Purpose

Fluorescent reporter for membrane voltage with improved brightness

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr1

Expression

Bacterial

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CL20_MSCV-NUP98::HOXA9-ires-GFP

Plasmid

#237484

Purpose

Express NUP98::HOXA9 in bicistronic retroviral vector

Depositor

Charles Mullighan

Publication

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::HOXA9 (HOXA9, NUP98 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_N-DYK_MTHFD1-NES

Plasmid

#133022

Purpose

NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Publication

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Tags

NES

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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MO70-mJP-4

Plasmid

#79598

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Publication

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH (AAV2)

Viral prep

#203840-AAV2

Purpose

Ready-to-use AAV2 particles produced from pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH (#203840). In addition to the viral particles, you will also receive purified pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH plasmid DNA. hSyn-driven expression of Kir2.1 and mScarlet-KASH. These AAV preparations are suitable purity for injection into animals.

Depositor

Ivo Spiegel

Publication

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Tags

mScarlett

Available since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only
pAAV-synapsin-HaloCaMP1a-EGFP (AAV1)

Viral prep

#138327-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-synapsin-HaloCaMP1a-EGFP (#138327). In addition to the viral particles, you will also receive purified pAAV-synapsin-HaloCaMP1a-EGFP plasmid DNA. Synapsin-driven HaloCaMP1a, a far-red calcium sensor, fused to EGFP. These AAV preparations are suitable purity for injection into animals.

Depositor

Eric Schreiter

Publication

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Promoter

Syn

Tags

EGFP

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only
RF6

Bacterial strain

#62074

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Publication

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCfB3052(gRNA X-4, XI-3, XII-5)

Plasmid

#73294

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

Plasmid

#172106

Purpose

Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Publication

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1mED

Plasmid

#35033

Depositor

Sheila Stewart

Publication

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1mWT

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

PT MUTANT E160D

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
eDrEGFR

Plasmid

#184000

Purpose

Optically controlled eDrEGFR expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Publication

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrEGFR (EGFR Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Gfi1b

Plasmid

#131611

Purpose

expression of mouse Gfi1b in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Publication

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Gfi1b (Gfi1b Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT6-G60A-FLAG

Plasmid

#102323

Purpose

Human SIRT6 G60A with FLAG tag for mammalian expression

Depositor

Hening Lin

Publication

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed glycine 60 to alanine

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBN260

Plasmid

#86722

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmyo-3::FLP D5

Expression

Worm

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBN278

Plasmid

#86724

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmec-7::FLP D5

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-DroshaS300E/S302D

Plasmid

#62531

Purpose

Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid

Depositor

Bharat Ramratnam

Publication

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to glutamic acid and serine 3…

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
PhyB-mCherry-SNF7

Plasmid

#51574

Purpose

Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Publication

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SNF7 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pJV0042

Plasmid

#205138

Purpose

Expression of 6xHis-EGFP-SnxA in bacteria

Depositor

Jason King

Publication

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

6xHis

Expression

Bacterial

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-SOX10

Plasmid

#134669

Purpose

Retroviral expression of human SOX10

Depositor

Marius Wernig

Publication

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

SRY-box transcription factor 10 (SOX10 Human)

Use

Retroviral

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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