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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,209 results for: GAN

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Showing: 1121 - 1140 of 7209 results

  4. pJW1354

    Plasmid
    #69490
    Purpose
    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms
    Depositor
    Jordan Ward
    Article
    Zhang et al Development. 2015 Nov 9. pii: dev.129635
    Insert
    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    Use
    CRISPR; Template to generate cassettes for -media…
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69490-plasmid-map-sequence-id-122661

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  5. pCZGY2850

    Plasmid
    #87758
    Purpose
    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.
    Depositor
    Andrew Chisholm
    Article
    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10
    Insert
    Pcol-19-PH-miniSOG(Q103L)
    Tags
    PH
    Expression
    Worm
    Mutation
    Q103L
    Promoter
    Pcol-19
    Available Since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    87758-plasmid-map-sequence-id-174819

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  9. ITGB3

    Plasmid
    #51921
    Purpose
    Expresses full-length Integrin beta-3 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGB3 (ITGB3 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 718, before CD4, bio and…
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51921-plasmid-map-sequence-id-79900

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  11. pJW2072

    Plasmid
    #154334
    Purpose
    Crispr repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154334-plasmid-map-sequence-id-318659

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  12. pPD158.87

    Plasmid
    #1709
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    sur5-10k, sur5tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1709-plasmid-map-sequence-id-315

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  14. pJW1584

    Plasmid
    #121055
    Purpose
    YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    YPET^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized YPET, and auxi…
    Expression
    Worm
    Available Since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121055-plasmid-map-sequence-id-235991

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  16. pcDNA3-Lyn-dPlcR

    Plasmid
    #181832
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-PlcR
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181832-plasmid-map-sequence-id-364041

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Showing: 1121 - 1140 of 7209 results