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peft-3::cas-9::tbb-2 3'UTR

Plasmid

#48960

Purpose

this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Insert

cas9

Use

CRISPR

Promoter

eef-1A.1 (eft-3)

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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DinoIII-arrO-N

Plasmid

#128143

Purpose

Dinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marina

Depositor

Senjie Lin

Article

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

Rifampin ADP-ribosylating transferase + potential promoter

Expression

Bacterial

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pKS2236

Plasmid

#37833

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

YFP

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pJA258

Plasmid

#21511

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::citrine(w introns)::tbb-2 3'UTR

Use

Multisite gateway

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pJL1121

Plasmid

#78188

Purpose

intestinal GFP expression by fasting in worms

Depositor

Junho Lee

Article

Jo et al Cell Metab. 2009 May;9(5):440-8. doi: 10

Insert

fil-2 (fil-2 Nematode)

Expression

Worm

Promoter

fil-2

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pID3.01B

Plasmid

#22720

Depositor

Geraldine Seydoux

Article

D'Agostino et al Dev Biol. 2006 Apr 1. 292(1):244-52.

Insert

unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’

Expression

Bacterial

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pAP997-1

Plasmid

#99500

Purpose

3XFlag::tagRFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagRFP::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2839

Plasmid

#87765

Purpose

Prgef-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Prgef-1-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Prgef-1

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGC146

Plasmid

#19613

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phsp-16.2::FLP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt

Expression

Worm

Promoter

hsp-16.2

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pID2.02

Plasmid

#26955

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’

Expression

Worm

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pAP975-1

Plasmid

#99498

Purpose

TEV::linker::tagBFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagBFP::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAP758-1

Plasmid

#99494

Purpose

TEV::eGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP546-2

Plasmid

#99476

Purpose

3XFlag::mNeptune::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::mNeptune::Myc

Expression

Bacterial

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2141

Plasmid

#87757

Purpose

Pcol-19-PH::miniSOG, expression of membrane targeted PH-miniSOG in epidermis.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pcol-19-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pcol-19

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFM032

Plasmid

#40076

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Type

Empty backbone

Tags

Dendra2 PAFP, S-peptide, and TEV protease cleavag…

Expression

Worm

Promoter

pie-1

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pMLS297

Plasmid

#73737

Purpose

SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

STOP codon + let-858 3'-UTR

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pNM3810

Plasmid

#154119

Purpose

mec-4 promoter driving QF in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::QF::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEL392

Plasmid

#52348

Purpose

GFP reporter for fli-1

Depositor

Erik Lundquist

Article

Lu et al BMC Dev Biol. 2008 May 16;8:54. doi: 10.

Insert

fli-1 (fli-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

fli-1

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pJRK86

Plasmid

#173743

Purpose

general AID::GFP expression

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::AID::GFP::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGC180

Plasmid

#19616

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(attR1-attR2)::FLP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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