We narrowed to 19,373 results for: LEA

1. pEMS1673

   Plasmid

   #29290

   Purpose

   Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple201 (SLC6A5 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEMS1136

   Plasmid

   #29056

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple198 (SLC6A4 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK215

   Plasmid

   #72427

   Purpose

   Acetobacter aceti 1023 gDNA

   Depositor

   T. J. Kappock

   Inserts

   succinyl-diaminopimelate desuccinylase
   2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
   acetylglutamate kinase
   ribosome biogenesis GTP-binding protein YsxC
   insertase
   ribonuclease P
   50S ribosomal protein L34
   hypothetical protein
   glyoxylase I
   hypothetical protein
   UDP-N-acetylglucosamine acyltransferase
   3-hydroxyacyl-ACP dehydratase
   UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
   

   Use

   Phage lambda cloning vector with genomic dna inse…

   Tags

   Expression

   Mutation

   missing C-terminal domain residues 277-391(ter) a…

   Promoter

   n/a

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Plxnb2(S)-AP-His

   Plasmid

   #72003

   Purpose

   Expresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxnb2 (Plxnb2 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH731-2µ-RLuc/minCFLuc

   Plasmid

   #40601

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-flex-taCasp3-TEVp

   Plasmid

   #45580

   Depositor

   Jim Wells, Nirao Shah

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Has Service

   AAV1 and AAV5

   Inserts

   Caspase 3 (CASP3 Synthetic, Human)
   Tobacco Etch Virus protease
   

   Use

   AAV; Adeno associated viral vector

   Tags

   Expression

   Mutation

   Linker replaced with a TEV protease cleavage site

   Promoter

   EF1a

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-N-Flag-ASC1

   Plasmid

   #75134

   Purpose

   Expresses N-terminal Flag-tagged ASC1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   PYD and CARD domain containing (Pycard Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F23S (please see depositor comments below)

   Promoter

   CMV

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRIG-myc-Sema4D-CD4

   Plasmid

   #51606

   Purpose

   expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone

   Depositor

   Suzanne Paradis

   Article

   Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

   Insert

   Sema4D/CD4-FLAG (Sema4d Human, Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and myc

   Expression

   Mammalian

   Mutation

   Sema4D amino acids 660-861 were replaced with CD4…

   Promoter

   RSV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pET21-10XHis-GST-HRV-dL5

   Plasmid

   #73214

   Purpose

   Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

   Insert

   EGFR (EGFR Schistosoma japonicum, Human)
   

   Use

   Tags

   10XHis-GST-HRV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT/TO/HIS/FLAG/HA-HNRNPU

    Plasmid

    #38068

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. hGFAP-Cre

    Plasmid

    #40591

    Purpose

    Mouse astrocyte expression of Cre

    Depositor

    Albee Messing

    Article

    Zhuo et al Genesis. 2001 Oct;31(2):85-94.

    Insert

    Cre recombinase (GFAP Human, Mouse, Phage)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    MP-1 fragment (mouse protamine 1 intron and polya…

    Expression

    Mammalian

    Mutation

    Construct contains a nuclear-targeted Cre recombi…

    Promoter

    hGFAP promoter

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-CIB1-mRFP1-MP

    Plasmid

    #58367

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Inserts

    CIB1[1-147] (CIB1 Mustard Weed)
    Camk2a[315-478] (Camk2a Rat)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-UbC-eGFP

    Plasmid

    #62518

    Purpose

    Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

    Depositor

    Jae Lee, Pantelis Tsoulfas

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cntn1-Fc-His

    Plasmid

    #72065

    Purpose

    Expresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn1 (Cntn1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid

    #64064

    Purpose

    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

    Depositor

    Andrew Carter

    Article

    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

    Insert

    Cytoplasmic dynein-2 (DYNC2H1 Human)
    

    Use

    Tags

    GFP, His tag, and ZZ tag

    Expression

    Insect

    Mutation

    Deleted amino acids 1-1090, extra C-terminal vali…

    Promoter

    Polyhedrin

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. mIFP24-LaminA-C-18

    Plasmid

    #56267

    Purpose

    Localization: Nuclear Envelope, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    LaminA (LMNA Human)
    

    Use

    Tags

    mIFP24

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

    Plasmid

    #70085

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

    Promoter

    SV40

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cntn2-Fc-His

    Plasmid

    #72066

    Purpose

    Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn2 (Cntn2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDN-D2irTN6kwh

    Plasmid

    #44724

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    htetR::NLS
    

    Use

    Synthetic Biology; Expression regulator/reporter;…

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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