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AKARet-cyto

Plasmid

#96897

Purpose

FLIM sensor for PKA activation in cytosol

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

FLIM Sensor for PKA Activity in Cytosol

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDS1928

Plasmid

#167284

Purpose

Expression plasmid for Candida albicans containing a red-shifted luciferase

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Inserts

red shifted luciferase
SAT1

Promoter

ACT1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pKM493

Plasmid

#140191

Purpose

ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

TEV-Flag-Gly4-eGFP

Tags

Flag + eGFP

Expression

Bacterial

Promoter

PGroEL

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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ePE2

Plasmid

#202029

Purpose

Prime Editing plasmid for tag insertion

Depositor

Pengcheng Wei

Article

Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

Insert

Cas9，

Use

CRISPR

Expression

Plant

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL198

Plasmid

#180472

Purpose

For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

Depositor

Peggy Lemaux

Article

Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

ZmCas9

Expression

Plant

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-BETAipi-ispA/dxs

Plasmid

#178302

Purpose

Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

ispA
dxs

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTH101

Plasmid

#84459

Purpose

pJB38-NWMN29-30 + SarA_P1-DsRed-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-DsRED from pTH1

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-SwiChRca-TS-EYFP

Plasmid

#55630

Purpose

Chloride Channel

Depositor

Karl Deisseroth

Article

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

CaMKIIa

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2186

Plasmid

#40386

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

WT aa1867-2186

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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Plk1 FRET sensor c-jun substrate

Plasmid

#45203

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Plk1 FRET sensor with c-jun substrate

Tags

CyPET, YPET

Expression

Mammalian

Promoter

T7

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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luxB:Venus+luxA/pRSETB Duet

Plasmid

#197582

Purpose

BRET lux luciferase with Venus for bacterial expression

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxBVenus

Use

E.coli expression

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJW1662

Plasmid

#112050

Purpose

C-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEU

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Inserts

IAA17
TIR1

Tags

HA

Expression

Yeast

Mutation

only amino acids 71-114

Promoter

ADH1 and None

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFS561

Plasmid

#242861

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetS promoter at its endogenous locus

Depositor

Nick Rhind

Article

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetS promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFS562

Plasmid

#242862

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locus

Depositor

Nick Rhind

Article

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW1 promoter

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFS564

Plasmid

#242864

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locus

Depositor

Nick Rhind

Article

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW3 promoter

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFS565

Plasmid

#242865

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locus

Depositor

Nick Rhind

Article

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW4 promoter

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pJKR-H-araC

Plasmid

#62563

Purpose

Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-IMX-DogTag

Plasmid

#113765

Purpose

Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

Depositor

Mark Howarth

Article

Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

Insert

IMX-DogTag

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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