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Addgene
Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer Immunotherapy.
Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, Wehrspaun C, Tabach Y, Sharp PA, Lu TK
Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 10.1016/j.cell.2017.09.049. Epub 2017 Oct 19. PubMed Article

Plasmids from Article

ID Plasmid Purpose
105171p119 (FUGW-UbCp-rtTA3)ubiquitin C promoter drives rtTA3 expression
105172pLN342 (FuGW-SSX1p-mKate2)SSX1 promoter drives mKate2 expression
105173pLN343 (FuGW-H2A1p-mKate2 )H2A1 promoter drives mKate2 expression
105174pLN351 (FuGW-S(YBX1)p-mKate2 )synthetic promoter YBX1 drives mKate2 expression
105175 pLN352 (FuGW-S(Elk1)p-mKate2)synthetic promoter Elk1 drives mKate2 experssion
105176pLN377 (FuGW-S(SP1)p-mKate2 )synthetic promoter SP1 drives mKate2 expression
105177pLN379 (FuGW-S(E2F1)p-mKate2)synthetic promoter E2F1 drives mKate2 expression
105178pLN380 (FuGW-S(CEBPB)p-mKate2)synthetic promoter CEBPB drives mKate2 expression
105179pLN381 (FuGW-S(NFY)p-mKate2)synthetic promoter NFY drives mKate2 expression
105180pLN382 (FuGW-S(HIF1A)p-mKate2 )synthetic promoter HIF1A drives mKate2 expression
105181pLN397 (FuGW-S(E2F1)p-Sponge7) Module 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)
105182pLN410 (FuGW-S(E2F1)p-ECFP sponge control)Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)
105183pLN426 (FuGW-G5p-mKate2)Module 3 - GAL4 synthetic promoter (with five binding sites) drives mKate2 expression
105184pLN428 (FuGW-G5p-mKate2-1Pe )Module 3 - GAL4 synthetic promoter (with five binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)
105185pLN429 (FuGW-G8p-mKate2)Module 3 - GAL4 synthetic promoter ( with eight binding sites) drives mKate2 expression
105186pLN431 (FuGW-G8p-mKate2-1Pe )Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)
105187pLN432 (FuGW-G14p-mKate2)Module 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2
105188pLN434 (FuGW-G14p-mKate2-1Pe )Module 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)
105189pLN466 (FuGW-S(cMYC)p-mKate2)synthetic promoter cMYC drives mKate2 expression
105190pLN472 (FuGW-G8p-STE-1Pe)Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of STE transcripts containing miR1-Bs(Pe)
105191pLN481 (FuGW-G8p-IL12-1Pe)Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)
105192pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe) Module 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)
105193pLN491 (FuGW-G8p-CCL21-1Pe)Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of CCL21 transcripts containing miR1-Bs(Pe)
105194pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)
105195pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)
105200pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)
105201pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)
105202pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)Module 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)
105203pLN553 (FuGW-S(MAFK)p-Sponge7)Module 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)
105204pLN592 (FuGW-S(USF1)p-mKate2)synthetic promoter USF1 drives mKate2 expression
105205pLN593 (FuGW-S(MAFK)p-mKate2)synthetic promoter MAFK drives mKate2 expression
105206pLN599 (pFuGW-hUbCp-hSTE1)ubiquitin C promoter drives STE expression
105207pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)
105209pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)
105210pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

Antibodies from Article