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EC71100

Plasmid

#154068

Purpose

Level 0 Golden Gate vector, pHvHSP17

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

pHvHSP17

Use

Standard cloning vector used by geneart for synth…

Mutation

All BsaI, Esp3I and BpiI restriction sites were r…

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1pr_v2-RT

Plasmid

#97088

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1pr_v2-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAB078d8

Plasmid

#79206

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBLO 64.1

Plasmid

#123120

Purpose

E. coli expression vector for active deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Promoter

NA

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP11H

Plasmid

#188063

Purpose

PROSS-designed versatile peroxidase 11H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

38 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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UKNP1.7.1

Plasmid

#97371

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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GST-TPX2(1-43)

Plasmid

#69107

Purpose

For bacterial expression of human TPX2(1-43aa) tagged with GST.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Targeting Protein for Xklp2 (TPX2 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-43 expressed.

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRS1422

Plasmid

#12548

Depositor

Robert Sobol

Article

Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

Insert

human Methylpurine DNA glycosylase (MPG Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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Pdgfra-T2A-H2B-eGFP donor

Plasmid

#113120

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Cx32-7

Plasmid

#55287

Purpose

Localization: Gap Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Cx32 (GJB1 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-NFAT5

Plasmid

#79506

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

NFAT5 (NFAT5 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro BRIT1

Plasmid

#16205

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

BRIT1 (MCPH1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pET17b-yebST

Plasmid

#175804

Purpose

Complementation of yebST knockout in E. coli K-12

Depositor

Gira Bhabha, Damian Ekiert, Ian Henderson

Article

Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

Insert

yebST

Tags

None

Expression

Bacterial

Mutation

silent mutations to introduce restriction sites

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCB

Plasmid

#162699

Purpose

Expresses pHnCB10 derived carboxysome operon and prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp6

Plasmid

#163590

Purpose

Expresses BMP-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp6 (Bmp6 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pVRb20_992

Plasmid

#49714

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf20_992

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Flag-YAP-S127/381A

Plasmid

#33069

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

S127A, S381A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pTMEM-PhoCl-Insert

Plasmid

#219674

Purpose

Caging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet

Depositor

Helge Ewers

Article

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Insert

TMEM-PhoCl-rtTA3

Tags

PhoCl

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVRa20_992

Plasmid

#49673

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF20_992

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSlug-4

Plasmid

#40648

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-4 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only