We narrowed to 27,844 results for: sta

1. pJG85

   Plasmid

   #89281

   Purpose

   Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

   Depositor

   Daniel Voytas

   Article

   Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRCP

   Plasmid

   #195862

   Purpose

   Encodes chromogenic protein (CP). CP inducible with rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Insert

   Chromogenic Protein
   

   Expression

   Bacterial

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. XE122 XDsh delta DIX-GFP-CS2+

   Plasmid

   #16787

   Depositor

   Randall Moon

   Article

   Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

   Insert

   Xdsh-delta DIX (dvl2.L Frog)
   

   Use

   Xenopus expression

   Tags

   gfp

   Mutation

   deletion of aa 1-159

   Available Since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIY-mETV3-Flag

   Plasmid

   #34579

   Depositor

   Peter Murray

   Article

   El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

   Insert

   Etv3 (Etv3 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG and IRES-YFP

   Expression

   Mammalian

   Available Since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pOBCol3.6-GFPtpz

   Plasmid

   #110211

   Purpose

   Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

   Depositor

   David Rowe

   Article

   Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

   Insert

   eGFPtopaz
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   3.6 kb rat type 1 collagen + first intron

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. px330-UFL1 sgRNA2

   Plasmid

   #134636

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA2 (UFL1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINK-TRABID (NZF1, aa 1-33)

   Plasmid

   #65437

   Purpose

   Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   TRABID (ZRANB1 Human)
   

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   only codes for amino acids 1-33 of TRABID, which …

   Promoter

   T7

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pAB085d

   Plasmid

   #79209

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ptet

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFAB815

   Plasmid

   #47854

   Purpose

   BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   rnpB T1 terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLTetO9

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-Flex-mRuby3-2A

    Plasmid

    #203846

    Purpose

    Neuron specific expression of a Cre-dependent mRuby3

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    mRuby3
    

    Use

    AAV

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pzyxin-emiRFP703

    Plasmid

    #136568

    Purpose

    Zyxin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    zyxin-emiRFP703
    

    Tags

    emiRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS SK mCherryROSAbsr

    Plasmid

    #54321

    Purpose

    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and BlasticidinS-resistance
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2SCb

    Plasmid

    #59385

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat2
    

    Use

    Template

    Mutation

    The 3’-end of the chloramphenicol resistance gene…

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. peCALWY-2

    Plasmid

    #22234

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-2 Zn(II) sensor (ATOX1 GFP's, Human)
    

    Expression

    Mammalian

    Mutation

    sequence identical to eCALWY-1, but linkerlength …

    Available Since

    Nov. 20, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pSAG1::CAS9-U6::sg290860-6

    Plasmid

    #59855

    Purpose

    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

    Depositor

    David Sibley

    Article

    Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

    Insert

    CRISPR sg290860-6
    

    Use

    CRISPR; ; toxoplasma gondii

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO h-PKD1 FLS (4107-4303)-GST

    Plasmid

    #83456

    Purpose

    Human PC1-CTp30 expression cassette with a GST-tag (678)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABE-Src-Rescue

    Plasmid

    #26983

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    c-Src (CSK Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pL0_EF-PtBle

    Plasmid

    #202180

    Purpose

    Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    ShBle expression cassette
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pBLO2 casX

    Plasmid

    #123122

    Purpose

    E. coli expression vector for CasX sgRNA

    Depositor

    Benjamin Oakes, Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX guide RNA
    

    Use

    CRISPR

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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