We narrowed to 15,988 results for: LEA

1. pmTurquoise2-NES

   Plasmid

   #36206

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   NES
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA421-Cas9tr

   Plasmid

   #138709

   Purpose

   Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

   Depositor

   Víctor de Lorenzo

   Article

   Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

   Insert

   tracrRNA-Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCVhyg-3xT7-nSREBP1a

   Plasmid

   #171030

   Purpose

   Expresses 3xT7-tagged nuclear form of SREBP1a in mammalian cells

   Depositor

   Kai Ge

   Article

   Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

   Insert

   nSREBP1a (Srebf1 Mouse)
   

   Use

   Retroviral

   Tags

   3xT7

   Expression

   Mammalian

   Mutation

   amino acids 1- 449

   Promoter

   5' LTR

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCoofy55

   Plasmid

   #122007

   Purpose

   Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His10-SumoStar (Life Sensors) and Multi2: dependi…

   Expression

   Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PARP15-c020

   Plasmid

   #173101

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP15 (PARP15 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N78-S267, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PARP15-c021

   Plasmid

   #173102

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP15 (PARP15 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   G287-N470, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVT-dCas9-mCherry

   Plasmid

   #174443

   Purpose

   in vitro transcription template for dCas9-mCherry

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Cas9 Endonuclease Dead
   

   Use

   In vitro transcription template

   Tags

   mCherry

   Promoter

   T7 SP6

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTAMAHISTEV_BauA

   Plasmid

   #128952

   Purpose

   expresses BauA in E.coli

   Depositor

   James Naismith

   Article

   Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

   Insert

   ferric acinetobactin receptor
   

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-66)

   Promoter

   T7

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGCDNsam-Hnf4a

   Plasmid

   #33002

   Depositor

   Atsushi Suzuki

   Article

   Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

   Insert

   hepatic nuclear factor 4 alpha (Hnf4a Mouse)
   

   Use

   Retroviral

   Available Since

   Feb. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpyCatcher002-oDi

    Plasmid

    #124661

    Purpose

    Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oDi
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid

    #216283

    Purpose

    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SpyCatcher003-TEVs-SpyTag003DA
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046768

    Plasmid

    #145763

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pIVT-dCas9-EGFP

    Plasmid

    #174442

    Purpose

    in vitro transcription template for dCas9-EGFP

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-Trpa1b-2A-mCherry

    Plasmid

    #106291

    Purpose

    Gateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherry

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Insert

    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway middle entry

    Tags

    2A-mCherry

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBTet_NAD-Snifit-nucl

    Plasmid

    #113915

    Purpose

    Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NAD-Snifit
    

    Expression

    Mammalian

    Promoter

    CMV TetO2

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpyCatcher002-oTet

    Plasmid

    #124663

    Purpose

    Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTet
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-mCherry-CRY2-NLS

    Plasmid

    #145259

    Purpose

    Mammalian Expression of Nuclear Control OptoConstruct with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid

    #193315

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    

    Tags

    4FLAG

    Expression

    Bacterial

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4046959

    Plasmid

    #145722

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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