We narrowed to 15,989 results for: LEA

1. pCSC-NES-RFP

   Plasmid

   #221812

   Purpose

   Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear export sequence (NES)

   Depositor

   Baojin Ding

   Article

   Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

   Insert

   NES-mCherry
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Prab-3::NLS::GCaMP6s

   Plasmid

   #68119

   Purpose

   Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

   Depositor

   Andrew Leifer

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

   Insert

   rab-3::NLS::GCaMP6s
   

   Expression

   Worm

   Promoter

   rab-3

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST14-Avitag-SpyCatcher002

   Plasmid

   #102828

   Purpose

   Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Avitag-SpyCatcher002
   

   Tags

   Avitag, GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LeGO-lnc-MONC

   Plasmid

   #86966

   Purpose

   Expresses lncRNA MONC (Alias: MIR99AHG; LINC00478)

   Depositor

   Jan-Henning Klusmann

   Article

   Emmrich et al Mol Cancer. 2014 Jul 15;13:171. doi: 10.

   Insert

   MONC isoform 6 (MIR99AHG Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAF

   Plasmid

   #104152

   Purpose

   Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

   Depositor

   Robert Craigie

   Article

   Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

   Insert

   Barrier-to-autointegration factor (BAF) (BANF1 Human)
   

   Tags

   N-terminal His tag with thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pre_II_CS3_7653_v2

   Plasmid

   #126855

   Purpose

   7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffold

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   II_CS3_7653_v2
   

   Use

   Synthetic Biology

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNES-iRFP-C1

   Plasmid

   #116863

   Purpose

   cytosolic iRFP

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   iRFP713
   

   Tags

   X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpyCatcher002-oHex

   Plasmid

   #124670

   Purpose

   Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oHex
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-SMVP-Cas9N-U6-gRNA M3

   Plasmid

   #80936

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA24N_6xHis-TEV-rpsA

    Plasmid

    #83585

    Purpose

    Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    rpsA (rpsA Escherichia coli)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    p-T5 Lac

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MCP-ADAR2-DD

    Plasmid

    #170124

    Purpose

    Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD-NES
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1a core promoter

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-UBC9-K14R-A129K

    Plasmid

    #228948

    Purpose

    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

    Depositor

    Marcin Suskiewicz

    Insert

    UBC9 (UBE2I Human)
    

    Tags

    His tag and thrombine cleavage site : MGHHHHHHSSG…

    Expression

    Bacterial

    Mutation

    changed Lysine 14 to Arginine, changed Alanine 12…

    Promoter

    T7-lac

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. mApple-hCOL1A1

    Plasmid

    #140113

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGE1109

    Plasmid

    #202773

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH2B-Electra1

    Plasmid

    #179478

    Purpose

    Nuclear labeling with blue fluorescent protein Electra1 fused to H2B

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    H2B-Electra1
    

    Tags

    Electra1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF6 VPS52-V5-6His

    Plasmid

    #175096

    Purpose

    V5 tagged VPS52 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

    Insert

    VPS52 (VPS52 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    N666D- please see depositor comment

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

    Plasmid

    #182009

    Purpose

    CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    IgKchL_HA_STf_myc_PDGFRtmb
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpyCatcher002-oPent

    Plasmid

    #124664

    Purpose

    Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oPent
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmScarlet-H_NES_C1

    Plasmid

    #85061

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. mVenus-hCOL1A1

    Plasmid

    #140111

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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