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M27 NacMS>luc

Plasmid

#17177

Depositor

Randall Moon

Article

Dorsky et al Genes Dev. 2000 Jan 15;14(2):158-62.

Insert

nacre promoter (mitfa Zebrafish)

Use

Luciferase

Tags

luciferase

Mutation

All three Tcf/Lef sites are mutated

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
EC.SurA.pTYB1.a21-428.L239R

Plasmid

#15957

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

surA (surA E. coli)

Tags

intein

Expression

Bacterial

Mutation

L239R

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pMIG small T H42Q

Plasmid

#9062

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen H42Q (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

H42Q. Renders J domain nonfunctional.

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pTH734-2µ-RLuc/stopCFLuc

Plasmid

#40609

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase (nonsense mutant)
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pTH753-CEN-RLuc/min346maxCFLuc

Plasmid

#38219

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first 346 codons of the original FLuc gene we…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pTH752-CEN-RLuc/min103maxCFLuc

Plasmid

#38218

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first 103 codons of the original FLuc gene we…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pTH751-CEN-RLuc/min53maxCFLuc

Plasmid

#38217

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first 53 codons of the original FLuc gene wer…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/myc-M1S46X

Plasmid

#87718

Purpose

Expresses myc-M1S46X spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; S46X

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/myc-M1S44L

Plasmid

#87717

Purpose

Expresses myc-M1S44L spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; S44L

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag MFF iso4 S172A

Plasmid

#74393

Purpose

Flag tagged human S172A MFF isoform 4 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser 172 Ala (numbering based on MFF isoform 1)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
Neo1.c-AP-His

Plasmid

#71965

Purpose

Expresses the extracellular region of the Neogenin 1, isoform c protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.c (Neo1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Neo1.h-AP-His

Plasmid

#71970

Purpose

Expresses the extracellular region of the Neogenin 1, isoform h protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.h (Neo1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pX68

Plasmid

#46857

Purpose

OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 A138V (TP53 Mouse, Human)

Expression

Mammalian

Mutation

p53 A138V

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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LK4-Ets2

Plasmid

#44606

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2 (Ets2 Mouse)

Expression

Mammalian

Promoter

human Beta-Actin

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
pLX-V5-TRAF2 S102A-puro

Plasmid

#44113

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Mutation

Serine 102 to Alanine

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
SPL10p::pCGTAG (SPL10p::NLS-GFP)

Plasmid

#27410

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 promoter (AT1G27370 Mustard Weed)

Mutation

SPL10 promoter transcriptionally fused to a nucle…

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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MALT1 (3BFO)

Plasmid

#25216

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

malt1.226.314 (MALT1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acid residues 226-314 of Isoform 2

Available Since

Aug. 12, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pGEM-T-easy Sox17b

Plasmid

#25782

Depositor

Edward De Robertis

Article

Wessely et al Development. 2000 May . 127(10):2053-62.

Insert

Sox 17b (sox17b.1 Frog)

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
A337C

Plasmid

#24432

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
pBABE-zeo small T 88

Plasmid

#10690

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 88 (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

Contains only the N-termial 88 amino acids

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only