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pLentiV2-Opti-sgGLRX5.2-puro

Plasmid

#257580

Purpose

sgRNA targeting GLRX5

Depositor

Kivanc Birsoy

Publication

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

GLRX5 (GLRX5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiV2-Opti-sgISCU.2-puro

Plasmid

#257578

Purpose

sgRNA targeting ISCU

Depositor

Kivanc Birsoy

Publication

Liu et al Science. 2023 Nov 17;382(6672):820-828.

Insert

ISCU (ISCU Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 (sgRNA); EFS (Cas9 + puroR)

Available since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pVB240306

Plasmid

#250660

Purpose

E. coli expression of the GSM domain of mouse ChREBPalpha (aa 43-207)

Depositor

Charles Brenner

Publication

Tiwari et al Nat Metab. 2025 Nov;7(11):2284-2299. doi

Insert

ChREBP alpha GSM domain (Mlxipl Mouse)

Tags

6xHis

Expression

Bacterial

Mutation

contains aa 43-307 only, with optimized E. coli c…

Promoter

T7

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mIRFP670nano3

Plasmid

#244107

Purpose

Bacterial expression of green glucose sensor with inert mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Tags

mIRFP670nano3

Expression

Bacterial

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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MBP-FUS-RGG3

Plasmid

#243672

Purpose

Expresses MBP-tagged FUS RGG3 domain in bacteria

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2021 Nov;28(11):923

Insert

FUS-RGG3 (FUS Human)

Tags

Maltose binding protein

Expression

Bacterial

Mutation

WT

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_006_Kan/ColEI_sfGFP

Plasmid

#235972

Purpose

Kanamycin/ColEI backbone with sfGFP placeholder for lvl2 assembly

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Kan/ColEI

Use

Synthetic Biology

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_2-3a_004_atpA_Promoter+5'UTR_Cr

Plasmid

#235876

Purpose

atpA promoter + 5'UTR Chlamydomonas reinhardtii 2-3a

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

atpA promoter + 5'UTR

Use

Synthetic Biology

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_005_Kan/ColEI_mScarlet-I

Plasmid

#235971

Purpose

Kanamycin/ColEI backbone with mScarlet-I placeholder for lvl2 assembly

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Kan/ColEI

Use

Synthetic Biology

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_2_064_Synthetic_promoter_08

Plasmid

#235817

Purpose

Synthetic promoter 08 based on Chlamydomonas rrn16 promoter

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Synthetic promoter 08

Use

Synthetic Biology

Mutation

none

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_3_001_5'UTR_placeholder

Plasmid

#235830

Purpose

Encodes a sfGFP cassette in the 5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_032_rbcL_5'UTR_full_Nt

Plasmid

#235851

Purpose

rbcL 5'UTR full Nicotiana tabacum

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

rbcL full 5'UTR Nt

Use

Synthetic Biology

Mutation

none

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.H348H.HaloTag

Plasmid

#244108

Purpose

Bacterial expression of green glucose sensor (high affinity) with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Tags

HaloTag

Expression

Bacterial

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_047_TMV_3'UTR_Tv

Plasmid

#235932

Purpose

TMV 3'UTR Tobacco Mosaic Virus

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

TMV 3'UTR

Use

Synthetic Biology

Available since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_4_023_ptxD-marker

Plasmid

#235899

Purpose

Phosphite oxidoreductase selection gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

ptxD-marker

Use

Synthetic Biology

Available since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_4_010_mCerulean3

Plasmid

#235897

Purpose

mCerulean3 reporter gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mCerulean3

Use

Synthetic Biology

Available since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_4_009_mVenus

Plasmid

#235896

Purpose

mVenus reporter gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mVenus

Use

Synthetic Biology

Available since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_4_033_Firefly_luciferase

Plasmid

#235902

Purpose

Firefly luciferase (red-shifted), codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Fluc

Use

Synthetic Biology

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_009_rbcL_3'UTR+rbcL_CDS

Plasmid

#235911

Purpose

rbcL 3'UTR + rbcL CDS Chlamydomonas reinhardtii

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

rbcL 3'UTR

Use

Synthetic Biology

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_3b_002_Placeholder

Plasmid

#235877

Purpose

Encodes a sfGFP cassette in the N-tag position used for placeholder cloning

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

N-tag placeholder

Use

Synthetic Biology

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_2-3_003_atpA_Promoter+5'UTR_Cr

Plasmid

#235873

Purpose

atpA promoter + 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

atpA promoter + 5'UTR

Use

Synthetic Biology

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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