We narrowed to 39,842 results for: eng
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Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPPromoterMSCVAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHC042 (∆phaCAB)
Plasmid#226208PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZ_P-AOX1-eGFP
Plasmid#126718Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterAOX1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-PDI
Plasmid#202486PurposeExpresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)
ExpressionBacterialPromoterEM7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
ExpressionBacterialPromotertrcAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Linear-GFP
Plasmid#226258PurposeProduction of linear RNA via IVTDepositorInsertEGFP
UseIn vitro transcriptionAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB
Plasmid#169550PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertcHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
ExpressionMammalianPromoterPPGKAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein promoter-less
Plasmid#118008PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, amilCP
UseSynthetic Biology; Escherichia coliAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2355_Tier3(PB)-iRFP_PuroR
Plasmid#169654PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
Plasmid#73404PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
CmCHI
ExpressionBacterialPromoterT7Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-eGFP
Plasmid#126717Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterGAPAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Circular-ic-CVB3-GFP
Plasmid#226260PurposeProduction of in situ circularizable RNA via IVTDepositorInsertEGFP
UseIn vitro transcriptionAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Circular-ic-EMCV-GFP
Plasmid#226259PurposeProduction of in situ circularizable RNA via IVTDepositorInsertEGFP
UseIn vitro transcriptionAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFNC-3
Plasmid#233296PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
ExpressionBacterialPromoterPEM7 and unknownAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-cTF
Plasmid#87743PurposeIPTG-inducible expression of ligase-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and cTF (chimeric transcription factor base…ExpressionBacterialPromoterT5-lacAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2340_Tier3(SB)-iRFP_Puro
Plasmid#169638PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2362_Tier3(SB)-rtTA-PuroR
Plasmid#169658PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHC039 (∆phcA)
Plasmid#226207PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.DepositorInsertHomology arms for PhcA
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits