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Plasmid#54468PurposeAn amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
UseTagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-1 was amplified via PCR, which removed an i…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
myc-tagged NRP1 (human)
Plasmid#213068PurposeExpress myc-NRP1 in mammalian cellsDepositorInsertneuropilin 1 (NRP1 Human)
UseTagsMycExpressionMammalianMutationN-terminal myc tag was introduced by a three-step…PromoterT7Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX20
Plasmid#169443PurposeUsed for guide cloning with PCRDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX52
Plasmid#169444PurposeUsed for guide cloning with PCRDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPL5617_pUG_PuroR
Plasmid#60928PurposePCR template vector for generating puromycin resistance cassette.DepositorInsertpuromycin N-Acetyltransferase
UseLoxp flanked deletion cassette templateTagsExpressionMutationPromoterTEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM342
Plasmid#71486PurposeContains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.DepositorInsertloxP-hyg-loxP cassette
UseSource of loxp-hyg-loxp for pcr amplification.TagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM343
Plasmid#71487PurposeContains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.DepositorInsertloxP-hyg-cat-loxP
UseSource of loxp-hyg-cat- loxp for pcr amplificatio…TagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSWt1-Dest
Plasmid#59764PurposePCR template for amplifying the attL1-em7-kanr-attL2-pA cassetteDepositorInsertattL1-em7-kanR-attL2-pA
UseTagsExpressionMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR1D2-donor
Plasmid#112314PurposeCRISPR donor plasmid to tag human transcription factor NR1D2 with GFPDepositorInsertNR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
UseCRISPRTagsExpressionMutationhg38:3:23977483:A>T, hg38:3:23977515:A>G, h…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
mUAV
Plasmid#102680PurposeMobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable partsDepositorInsertJ23106:B0034:amilCP:rrnBT1-T7TE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP2375
Plasmid#127581PurposeTagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and NAT resistance geneDepositorInsertPtetO7.1_Flagtag_8Glycine linker
UseSynthetic Biology; Pcr based taggingTagsFlag tag, 8glycine linkerExpressionMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2350
Plasmid#127580PurposeTagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance geneDepositorInsertPtetO7.1_Flagtag_8Glycine linker
UseSynthetic Biology; Pcr based taggingTagsFlag tag, 8glycine linkerExpressionMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPkan-S2
Plasmid#61601PurposePCR template for en passant (two-step Red-mediated) mutagenesisDepositorInsertKana_I-SceI
UseTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEPkan-S
Plasmid#41017PurposePCR template for en passant (two-step Red-mediated) mutagenesisDepositorInsertKana_I-SceI
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pATF4-donor
Plasmid#113805PurposeCRISPR donor plasmid to tag human transcription factor ATF4 with GFPDepositorInsertATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
UseCRISPRTagsExpressionMutationrs140428747PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA CRU HISMX
Plasmid#131164PurposePCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneUseTagsCRUExpressionYeastMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8739
Plasmid#126905PurposeTemplate for PCR amplification of XdCrtYBDepositorInsertXdCrtYB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8740
Plasmid#126906PurposeTemplate for PCR amplification of XdCrtIDepositorInsertXdCrtI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8741
Plasmid#126907PurposeTemplate for PCR amplification of XdCrtEDepositorInsertXdCrtE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only